LOCUS       sequence-111617-         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes yeast enhanced GFP under control of minimal pCYC1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Farzadfard F, Perli SD, Lu TK
  TITLE     Tunable and Multifunctional Eukaryotic Transcription Factors Based 
            on CRISPR/Cas.
  JOURNAL   ACS Synth Biol. 2013 Sep 11.
  PUBMED    23977949
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2013 Sep 11."
COMMENT     Sequence Label: pCYC1m_yeGFP
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..22
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     7..23
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ccccctcgag gtcgacggta tcgataagct tgatatcgaa ttcctgcagc ccgggtactg
       61 tatgtacata cagtaggatc ctatggcatg catgtgctct gtatgtatat aaaactcttg
      121 ttttcttctt ttctctaaat attctttcct tatacattag gacctttgca gcataaatta
      181 ctatacttct atagacacac aaacacaaat acacacacta atctagataa aaaatgtcta
      241 aaggtgaaga attattcact ggtgttgtcc caattttggt tgaattagat ggtgatgtta
      301 atggtcacaa attttctgtc tccggtgaag gtgaaggtga tgctacttac ggtaaattga
      361 ccttaaaatt tatttgtact actggtaaat tgccagttcc atggccaacc ttagtcacta
      421 ctttcggtta tggtgttcaa tgttttgcga gatacccaga tcatatgaaa caacatgact
      481 ttttcaagtc tgccatgcca gaaggttatg ttcaagaaag aactattttt ttcaaagatg
      541 acggtaacta caagaccaga gctgaagtca agtttgaagg tgatacctta gttaatagaa
      601 tcgaattaaa aggtattgat tttaaagaag atggtaacat tttaggtcac aaattggaat
      661 acaactataa ctctcacaat gtttacatca tggctgacaa acaaaagaat ggtatcaaag
      721 ttaacttcaa aattagacac aacattgaag atggttctgt tcaattagct gaccattatc
      781 aacaaaatac tccaattggt gatggtccag tcttgttacc agacaaccat tacttatcca
      841 ctcaatctgc cttatccaaa gatccaaacg a
//
