LOCUS       sequence-111621-        1049 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  harbours marker genes HIS3 and URA3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Mulleder M, Campbell K, Matsarskaia O, Eckerstorfer F, Ralser M
  TITLE     Saccharomyces cerevisiae single-copy plasmids for auxotrophy 
            compensation, multiple marker selection, and for designing 
            metabolically cooperating communities.
  JOURNAL   F1000Res. 2016 Sep 20;5:2351. eCollection 2016.
  PUBMED    27830062
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "F1000Res.";
            date: "2016-09-20"; volume: "5"; pages: "2351. eCollection 2016"
COMMENT     Sequence Label: pHU
FEATURES             Location/Qualifiers
     source          1..1049
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(18..40)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     140..159
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        306..493
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
ORIGIN
        1 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
       61 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcggacgt
      121 cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccataaattc
      181 ccgttttaag agcttggtga gcgctaggag tcactgccag gtatcgtttg aacacggcat
      241 tagtcaggga agtcataaca cagtcctttc ccgcaatttt ctttttctat tactcttggc
      301 ctcctctagt acactctata tttttttatg cctcggtaat gattttcatt tttttttttc
      361 cacctagcgg atgactcttt ttttttctta gcgattggca ttatcacata atgaattata
      421 cattatataa agtaatgtga tttcttcgaa gaatatacta aaaaatgagc aggcaagata
      481 aacgaaggca aagatgacag agcagaaagc cctagtaaag cgtattacaa atgaaaccaa
      541 gattcagatt gcgatctctt taaagggtgg tcccctagcg atagagcact cgatcttccc
      601 agaaaaagag gcagaagcag tagcagaaca ggccacacaa tcgcaagtga ttaacgtcca
      661 cacaggtata gggtttctgg accatatgat acatgctctg gccaagcatt ccggctggtc
      721 gctaatcgtt gagtgcattg gtgacttaca catagacgac catcacacca ctgaagactg
      781 cgggattgct ctcggtcaag cttttaaaga ggccctaggg gccgtgcgtg gagtaaaaag
      841 gtttggatca ggatttgcgc ctttggatga ggcactttcc agagcggtgg tagatctttc
      901 gaacaggccg tacgcagttg tcgaacttgg tttgcaaagg gagaaagtag gagatctctc
      961 ttgcgagatg atcccgcatt ttcttgaaag ctttgcagag gctagcagaa ttaccctcca
     1021 cgttgattgt ctgcgaggca agaatgatc
//
