LOCUS       sequence-111687-        9663 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tol2-based Wnt signal reporter plasmid (expresses NLS-ONL).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9663)
  AUTHORS   Takai A, Nakano M, Saito K, Haruno R, Watanabe TM, Ohyanagi T, Jin 
            T, Okada Y, Nagai T
  TITLE     Expanded palette of Nano-lanterns for real-time multicolor 
            luminescence imaging.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):4352-6. doi: 
            10.1073/pnas.1418468112. Epub 2015 Mar 23.
  PUBMED    25831507
REFERENCE   2  (bases 1 to 9663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1418468112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-04-7- 7"; volume: "112"; issue: "14"; pages: "4352-6"
COMMENT     Sequence Label: pT2-7xTcf-NLS-ONL
FEATURES             Location/Qualifiers
     source          1..9663
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     623..642
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(655..666,667..854)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     polyA_signal    2123..2171
                     /note="synthetic polyadenylation signal"
     primer_bind     2346..2370
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2460..2480
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2484..2504
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2508..2528
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2541..3194
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
     CDS             2541..3194
                     /codon_start=1
                     /product="humanized monomeric Kusabira-Orange fluorescent 
                     protein"
                     /label=hmKO
                     /note="mammalian codon-optimized"
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
     polyA_signal    4187..4411
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4458..4481)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4855..4874
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4939..5538
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4939..4958)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5435..5455
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    5625..5849
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5877..5893)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5901..5917
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5925..5931,5932..5949,5950..5955))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5970..5991
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    join(7307..7444,7445..7456)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     promoter        complement(7475..7493)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7514..7530)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7538..7554
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7562..7568,7569..7586,7587..7592))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7607..7628
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7745..7762)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7916..8504)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7996..8015)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8675..9466,9467..9535))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9298..9317
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9536..9640)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtaccagagg
      661 tgtaaagtac ttgagtaatt ttacttgatt actgtactta agtattattt ttggggattt
      721 ttactttact tgagtacaat taaaaatcaa tacttttact tttacttaat tacatttttt
      781 tagaaaaaaa agtacttttt actccttaca attttattta cagtcaaaaa gtacttattt
      841 tttggagatc acttgtcgag tagggataac agggtaatga tcccccgggc tgcagtcgcg
      901 acgcgtccta gagggacagc ccccccccaa agcccccagg gatgtaatta cgtccctccc
      961 ccgctagggg gcagcagcga gccgcccggg gctccgctcc ggtccggcgc tccccccgca
     1021 tccccgagcc ggcagcgtgc ggggacagcc cgggcacggg gaaggtggca cgggatcgct
     1081 ttcctctgaa cgcttctcgc tgctctttga gcctgcagac acctgggggg atacggggaa
     1141 aaagctttag gctgaaagag agatttagaa tgacagaatc atagaacggc ctgggttgca
     1201 aaggagcaca gtgctcatcc agatccaacc ccctgctatg tgcagggtca tcaaccagca
     1261 gcccaggctg cccagagcca catccagcct ggccttgaat gcctgcaggg atggggcatc
     1321 cacagcctcc ttgggcaacc tgttcagtgc gtcaccaccc tctgggggaa aaactgcctc
     1381 ctcatatcca acccaaacct cccctgtctc agtgtaaagc cattccccct tgtcctatca
     1441 agggggagtt tgctgtgaca ttgttggtct ggggtgacac atgtttgcca attcagtgca
     1501 tcacggagag gcagatcttg gggataagga agtgcaggac agcatggacg tgggacatgc
     1561 aggtgttgag ggctctggga cactctccaa gtcacagcgt tcagaacagc cttaaggata
     1621 agaagatagg atagaaggac aaagagcaag ttaaaaccca gcatggagag gagcacaaaa
     1681 aggccacaga cactgctggt ccctgtgtct gagcctgcat gtttgatggt gtctggatgc
     1741 aagcagaagg ggtggaagag cttgcctgga gagatacagc tgggtcagta ggactgggac
     1801 aggcagctgg agaattgcca tgtagatgtt catacaatcg tcaaatcatg aaggctggaa
     1861 aagccctcca agatccccaa gaccaacccc aacccaccca ccgtgcccac tggccatgtc
     1921 cctcagtgcc acatccccac agttcttcat cacctccagg gacggtgacc cccccacctc
     1981 cgtgggcagc tgtgccactg cagcaccgct ctttggagaa ggtaaatctt gctaaatcca
     2041 gcccgaccct cccctggcac aacgtaaggc cattatctct catccaactc caggacggag
     2101 tcagtgagga tggggctcta ggaataaaat atctttattt tcattacatc tgtgtgttgg
     2161 ttttttgtgt ggtcgacggt accgagctct tacgcgagat caaagggggt aagatcaaag
     2221 ggggtaagat caaaggggcg cgagatcaaa gggggtaaga tcaaaggggg taagatcaaa
     2281 gggggtaaga tcaaaggggc gcgcccgcgt gctaggcgtg tacggtggga ggcctatata
     2341 agcagagctc gtttagtgaa ccgtcagatc gcatcgattc gaattccagc ttggcattcc
     2401 ggtactgttg gtaaaaagct tggcattccg gtactgttgg taaagccacc atgctggtac
     2461 caaaaaagaa gagaaaggtt gtaccaaaaa agaagagaaa ggttgtacca aaaaagaaga
     2521 gaaaggtttt cgaagggccc atggtgagcg tgatcaagcc cgagatgaag atgaggtact
     2581 acatggacgg ctccgtcaat gggcatgagt tcacaatcga gggtgagggc acaggcagac
     2641 cttacgaggg acatcaggag atgacactgc gcgtcacaat ggccgagggc gggccaatgc
     2701 ctttcgcctt cgacctggtg tcccacgtgt tctgttacgg ccacagagtt tttactaaat
     2761 atccagaaga gatcccagac tatttcaagc aggcctttcc tgagggcctg tcctgggaga
     2821 ggtccctgga gttcgaggac ggcggctccg cctccgtgag cgcccacatc agcctgaggg
     2881 gcaacacctt ctaccacaag tccaagttca ccggcgtgaa cttccccgcc gacggcccca
     2941 tcatgcagaa ccagagcgtg gactgggagc cctccaccga gaagatcacc gccagcgacg
     3001 gcgtgctgaa gggcgacgtg accatgtacc tgaagctgga gggcggcggc aaccacaagt
     3061 gccagatgaa gaccacctac aaggccgcca aggagatcct ggagatgccc ggcgaccact
     3121 acatcggcca caggctggtg aggaagaccg agggcaacat caccgagcag gtggaggacg
     3181 ccgtggccca ctccggtacc aaggtgtacg accccgagca acgcaaacgc atgatcactg
     3241 ggcctcagtg gtgggctcgc tgcaagcaaa tgaacgtgct ggactccttc atcaactact
     3301 atgattccga gaagcacgcc gagaacgccg tgatttttct gcatggtaac gctacctcca
     3361 gctacctgtg gaggcacgtc gtgcctcaca tcgagcccgt ggctagatgc atcatccctg
     3421 atctgatcgg aatgggtaag tccggcaaga gcgggaatgg ctcatatcgc ctcctggatc
     3481 actacaagta cctcaccgct tggttcgagc tgctgaacct tccaaagaaa atcatctttg
     3541 tgggccacga ctgggggagc gctctggcct ttcactacgc ctacgagcac caagacagga
     3601 tcaaggccat cgtccatatg gagagtgtcg tggacgtgat cgagtcctgg atggggtggc
     3661 ctgacatcga ggaggagctg gccctgatca agagcgaaga gggcgagaaa atggtgcttg
     3721 agaataactt cttcgtcgag accctgttgc caagcaagat catgcggaaa ctggagcctg
     3781 aggagttcgc tgcctacctg gagccattca aggagaaggg cgaggttaga cggcctaccc
     3841 tctcctggcc tcgcgagatc cctctcgtta agggaggcaa gcccgacgtc gtccagattg
     3901 tccgcaacta caacgcctac cttcgggcca gcgacgatct gcctaagctg ttcatcgagt
     3961 ccgaccctgg gttcttttcc aacgctattg tcgagggagc taagaagttc cctaacaccg
     4021 agttcgtgaa ggtgaagggc ctccacttcc tccaggagga cgctccagat gaaatgggta
     4081 agtacatcaa gagcttcgtg gagcgcgtgc tgaagaacga gcagtaatct agagcggccg
     4141 ctcgataagc ttgatcgatg atctagagct cgctgatcag cctcgactgt gccttctagt
     4201 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     4261 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     4321 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     4381 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac ctgcagcttt
     4441 tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa
     4501 gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct
     4561 ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc
     4621 cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc
     4681 agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc
     4741 agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc
     4801 tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct
     4861 gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt
     4921 cgacctgtgg ccacaaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     4981 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     5041 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     5101 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     5161 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     5221 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     5281 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     5341 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     5401 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     5461 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     5521 cgcaagcccg gtgcctgacg cccgccccac gacccgcagc gcccgaccga aaggagcgca
     5581 cgaccccatg catcgatgat ctagagctcg ctgatcagcc tcgactgtgc cttctagttg
     5641 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     5701 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     5761 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     5821 gcatgctggg gatgcggtgg gctctatggc tgcagcccaa gcttggcgta atcatggtca
     5881 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     5941 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     6001 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agcggatccc tagagggaca
     6061 gccccccccc aaagccccca gggatgtaat tacgtccctc ccccgctagg gggcagcagc
     6121 gagccgcccg gggctccgct ccggtccggc gctccccccg catccccgag ccggcagcgt
     6181 gcggggacag cccgggcacg gggaaggtgg cacgggatcg ctttcctctg aacgcttctc
     6241 gctgctcttt gagcctgcag acacctgggg ggatacgggg aaaaagcttt aggctgaaag
     6301 agagatttag aatgacagaa tcatagaacg gcctgggttg caaaggagca cagtgctcat
     6361 ccagatccaa ccccctgcta tgtgcagggt catcaaccag cagcccaggc tgcccagagc
     6421 cacatccagc ctggccttga atgcctgcag ggatggggca tccacagcct ccttgggcaa
     6481 cctgttcagt gcgtcaccac cctctggggg aaaaactgcc tcctcatatc caacccaaac
     6541 ctcccctgtc tcagtgtaaa gccattcccc cttgtcctat caagggggag tttgctgtga
     6601 cattgttggt ctggggtgac acatgtttgc caattcagtg catcacggag aggcagatct
     6661 tggggataag gaagtgcagg acagcatgga cgtgggacat gcaggtgttg agggctctgg
     6721 gacactctcc aagtcacagc gttcagaaca gccttaagga taagaagata ggatagaagg
     6781 acaaagagca agttaaaacc cagcatggag aggagcacaa aaaggccaca gacactgctg
     6841 gtccctgtgt ctgagcctgc atgtttgatg gtgtctggat gcaagcagaa ggggtggaag
     6901 agcttgcctg gagagataca gctgggtcag taggactggg acaggcagct ggagaattgc
     6961 catgtagatg ttcatacaat cgtcaaatca tgaaggctgg aaaagccctc caagatcccc
     7021 aagaccaacc ccaacccacc caccgtgccc actggccatg tccctcagtg ccacatcccc
     7081 acagttcttc atcacctcca gggacggtga cccccccacc tccgtgggca gctgtgccac
     7141 tgcagcaccg ctctttggag aaggtaaatc ttgctaaatc cagcccgacc ctcccctggc
     7201 acaacgtaag gccattatct ctcatccaac tccaggacgg agtcagtgag gatggggctc
     7261 tagccggacc gacgcgtctc gatagggata acagggtaat tgatccaata ctcaagtaca
     7321 attttaatgg agtacttttt tacttttact caagtaagat tctagccaga tacttttact
     7381 tttaattgag taaaattttc cctaagtact tgtactttca cttgagtaaa atttttgagt
     7441 actttttaca cctctggagc tccagctttt gttcccttta gtgagggtta atttcgagct
     7501 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     7561 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     7621 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     7681 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     7741 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     7801 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     7861 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     7921 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     7981 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     8041 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     8101 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     8161 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     8221 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     8281 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     8341 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     8401 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     8461 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     8521 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     8581 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     8641 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     8701 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     8761 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     8821 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     8881 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     8941 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     9001 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     9061 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     9121 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     9181 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     9241 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     9301 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     9361 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     9421 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     9481 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     9541 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     9601 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     9661 cac
//
