LOCUS       sequence-111688-        9867 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tol2-based Wnt signal reporter plasmid (expresses NLS-YNL-CP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9867)
  AUTHORS   Takai A, Nakano M, Saito K, Haruno R, Watanabe TM, Ohyanagi T, Jin 
            T, Okada Y, Nagai T
  TITLE     Expanded palette of Nano-lanterns for real-time multicolor 
            luminescence imaging.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):4352-6. doi: 
            10.1073/pnas.1418468112. Epub 2015 Mar 23.
  PUBMED    25831507
REFERENCE   2  (bases 1 to 9867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1418468112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-04-7- 7"; volume: "112"; issue: "14"; pages: "4352-6"
COMMENT     Sequence Label: pT2-7xTcf-NLS-YNL-CP
FEATURES             Location/Qualifiers
     source          1..9867
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     623..642
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(655..666,667..854)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     polyA_signal    2123..2171
                     /note="synthetic polyadenylation signal"
     primer_bind     2346..2370
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2460..2480
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2484..2504
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2508..2528
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(2541..2543,2544..2546,2547..3059)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(2586..2607)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2847..2866)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3194..3215
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4158..4205
                     /codon_start=1
                     /product="non-ORF yeast peptide conferring 
                     ubiquitin-dependent degradation"
                     /label=hCL1
                     /note="human codon-optimized"
                     /translation="ACKNWFSSLSHFVIHL"
     CDS             4209..4328
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     polyA_signal    4391..4615
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4662..4685)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5059..5078
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5143..5742
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5143..5162)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5639..5659
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    5829..6053
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6081..6097)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6105..6121
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6129..6135,6136..6153,6154..6159))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6174..6195
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    join(7511..7648,7649..7660)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     promoter        complement(7679..7697)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7718..7734)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7742..7758
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7766..7772,7773..7790,7791..7796))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7811..7832
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7949..7966)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8120..8708)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8200..8219)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8879..9670,9671..9739))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9502..9521
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9740..9844)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtaccagagg
      661 tgtaaagtac ttgagtaatt ttacttgatt actgtactta agtattattt ttggggattt
      721 ttactttact tgagtacaat taaaaatcaa tacttttact tttacttaat tacatttttt
      781 tagaaaaaaa agtacttttt actccttaca attttattta cagtcaaaaa gtacttattt
      841 tttggagatc acttgtcgag tagggataac agggtaatga tcccccgggc tgcagtcgcg
      901 acgcgtccta gagggacagc ccccccccaa agcccccagg gatgtaatta cgtccctccc
      961 ccgctagggg gcagcagcga gccgcccggg gctccgctcc ggtccggcgc tccccccgca
     1021 tccccgagcc ggcagcgtgc ggggacagcc cgggcacggg gaaggtggca cgggatcgct
     1081 ttcctctgaa cgcttctcgc tgctctttga gcctgcagac acctgggggg atacggggaa
     1141 aaagctttag gctgaaagag agatttagaa tgacagaatc atagaacggc ctgggttgca
     1201 aaggagcaca gtgctcatcc agatccaacc ccctgctatg tgcagggtca tcaaccagca
     1261 gcccaggctg cccagagcca catccagcct ggccttgaat gcctgcaggg atggggcatc
     1321 cacagcctcc ttgggcaacc tgttcagtgc gtcaccaccc tctgggggaa aaactgcctc
     1381 ctcatatcca acccaaacct cccctgtctc agtgtaaagc cattccccct tgtcctatca
     1441 agggggagtt tgctgtgaca ttgttggtct ggggtgacac atgtttgcca attcagtgca
     1501 tcacggagag gcagatcttg gggataagga agtgcaggac agcatggacg tgggacatgc
     1561 aggtgttgag ggctctggga cactctccaa gtcacagcgt tcagaacagc cttaaggata
     1621 agaagatagg atagaaggac aaagagcaag ttaaaaccca gcatggagag gagcacaaaa
     1681 aggccacaga cactgctggt ccctgtgtct gagcctgcat gtttgatggt gtctggatgc
     1741 aagcagaagg ggtggaagag cttgcctgga gagatacagc tgggtcagta ggactgggac
     1801 aggcagctgg agaattgcca tgtagatgtt catacaatcg tcaaatcatg aaggctggaa
     1861 aagccctcca agatccccaa gaccaacccc aacccaccca ccgtgcccac tggccatgtc
     1921 cctcagtgcc acatccccac agttcttcat cacctccagg gacggtgacc cccccacctc
     1981 cgtgggcagc tgtgccactg cagcaccgct ctttggagaa ggtaaatctt gctaaatcca
     2041 gcccgaccct cccctggcac aacgtaaggc cattatctct catccaactc caggacggag
     2101 tcagtgagga tggggctcta ggaataaaat atctttattt tcattacatc tgtgtgttgg
     2161 ttttttgtgt ggtcgacggt accgagctct tacgcgagat caaagggggt aagatcaaag
     2221 ggggtaagat caaaggggcg cgagatcaaa gggggtaaga tcaaaggggg taagatcaaa
     2281 gggggtaaga tcaaaggggc gcgcccgcgt gctaggcgtg tacggtggga ggcctatata
     2341 agcagagctc gtttagtgaa ccgtcagatc gcatcgattc gaattccagc ttggcattcc
     2401 ggtactgttg gtaaaaagct tggcattccg gtactgttgg taaagccacc atgctggtac
     2461 caaaaaagaa gagaaaggtt gtaccaaaaa agaagagaaa ggttgtacca aaaaagaaga
     2521 gaaaggtttt cgaagggccc atggtgagca agggcgagga gctgttcacc ggggtggtgc
     2581 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     2641 gcgagggcga tgccacctac ggcaagctga ccctgaagct gatctgcacc accggcaagc
     2701 tgcccgtgcc ctggcccacc ctcgtgacca ccctgggcta cggcctgcag tgcttcgccc
     2761 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     2821 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     2881 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     2941 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
     3001 ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg
     3061 acggcggcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     3121 tgctgctgcc cgacaaccac tacctgagct accagtccaa gctgagcaaa gaccccaacg
     3181 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggggt accaaggtgt
     3241 acgaccccga gcagaggaag aggatgatca ccggccccca gtggtgggcc aggtgcaagc
     3301 agatgaacgt gctggacagc ttcatcaact actacgacag cgagaagcac gccgagaacg
     3361 ccgtgatctt cctgcacggc aacgccacta gcagctacct gtggaggcac gtggtgcccc
     3421 acatcgagcc cgtggccagg tgcatcatcc ccgatctgat cggcatgggc aagagcggca
     3481 agagcggcaa cggcagctac aggctgctgg accactacaa gtacctgacc gcctggttcg
     3541 agcttctgaa cctgcccaag aagatcatct tcgtgggcca cgactggggc gccgccctgg
     3601 ccttccacta cgcctacgag caccaggaca ggatcaaggc catcgtgcac atggagagcg
     3661 tggtggacgt gatcgagagc tgggacgagt ggccagacat cgaggaggac atcgccctga
     3721 tcaagagcga ggagggcgag aagatggtgc tggagaacaa cttcttcgtg gagaccgtgc
     3781 tgcccagcaa gatcatgaga aagctggagc ccgaggagtt cgccgcctac ctggagccct
     3841 tcaaggagaa gggcgaggtg agaagaccca ccctgagctg gcccagagag atccccctgg
     3901 tgaagggcgg caagcccgac gtggtgcaga tcgtgagaaa ctacaacgcc tacctgagag
     3961 ccagcgacga cctgcccaag ctgttcatcg agggcgaccc cggcttcttc agcaacgcca
     4021 tcgtggaggg cgccaagaag ttccccaaca ccgagttcgt gaaggtgaag ggcctgcact
     4081 tcctccagga ggacgccccc gacgagatgg gcaagtacat caagagcttc gtggagagag
     4141 tgctgaagaa cgagcaggct tgcaagaact ggttcagtag cttaagccac tttgtgatcc
     4201 accttaacag ccacggcttc cctcccgagg tggaggagca ggccgccggc accctgccca
     4261 tgagctgcgc ccaggagagc ggcatggata gacaccctgc tgcttgcgcc agcgccagga
     4321 tcaacgtcta atctagagcg gccgctcgat aagcttgatc gatgatctag agctcgctga
     4381 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     4441 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     4501 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     4561 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     4621 gaggcggaaa gaacctgcag cttttcccaa ggcagtctgg agcatgcgct ttagcagccc
     4681 cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg
     4741 gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc
     4801 tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag
     4861 tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag
     4921 gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg
     4981 gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa
     5041 ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct
     5101 cctcttcctc atctccgggc ctttcgacct gtggccacaa ccatgaccga gtacaagccc
     5161 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
     5221 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
     5281 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     5341 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     5401 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     5461 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     5521 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     5581 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     5641 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     5701 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gacgcccgcc ccacgacccg
     5761 cagcgcccga ccgaaaggag cgcacgaccc catgcatcga tgatctagag ctcgctgatc
     5821 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     5881 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     5941 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     6001 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggctgcagc
     6061 ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     6121 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     6181 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     6241 tgccagcgga tccctagagg gacagccccc ccccaaagcc cccagggatg taattacgtc
     6301 cctcccccgc tagggggcag cagcgagccg cccggggctc cgctccggtc cggcgctccc
     6361 cccgcatccc cgagccggca gcgtgcgggg acagcccggg cacggggaag gtggcacggg
     6421 atcgctttcc tctgaacgct tctcgctgct ctttgagcct gcagacacct ggggggatac
     6481 ggggaaaaag ctttaggctg aaagagagat ttagaatgac agaatcatag aacggcctgg
     6541 gttgcaaagg agcacagtgc tcatccagat ccaaccccct gctatgtgca gggtcatcaa
     6601 ccagcagccc aggctgccca gagccacatc cagcctggcc ttgaatgcct gcagggatgg
     6661 ggcatccaca gcctccttgg gcaacctgtt cagtgcgtca ccaccctctg ggggaaaaac
     6721 tgcctcctca tatccaaccc aaacctcccc tgtctcagtg taaagccatt cccccttgtc
     6781 ctatcaaggg ggagtttgct gtgacattgt tggtctgggg tgacacatgt ttgccaattc
     6841 agtgcatcac ggagaggcag atcttgggga taaggaagtg caggacagca tggacgtggg
     6901 acatgcaggt gttgagggct ctgggacact ctccaagtca cagcgttcag aacagcctta
     6961 aggataagaa gataggatag aaggacaaag agcaagttaa aacccagcat ggagaggagc
     7021 acaaaaaggc cacagacact gctggtccct gtgtctgagc ctgcatgttt gatggtgtct
     7081 ggatgcaagc agaaggggtg gaagagcttg cctggagaga tacagctggg tcagtaggac
     7141 tgggacaggc agctggagaa ttgccatgta gatgttcata caatcgtcaa atcatgaagg
     7201 ctggaaaagc cctccaagat ccccaagacc aaccccaacc cacccaccgt gcccactggc
     7261 catgtccctc agtgccacat ccccacagtt cttcatcacc tccagggacg gtgacccccc
     7321 cacctccgtg ggcagctgtg ccactgcagc accgctcttt ggagaaggta aatcttgcta
     7381 aatccagccc gaccctcccc tggcacaacg taaggccatt atctctcatc caactccagg
     7441 acggagtcag tgaggatggg gctctagccg gaccgacgcg tctcgatagg gataacaggg
     7501 taattgatcc aatactcaag tacaatttta atggagtact tttttacttt tactcaagta
     7561 agattctagc cagatacttt tacttttaat tgagtaaaat tttccctaag tacttgtact
     7621 ttcacttgag taaaattttt gagtactttt tacacctctg gagctccagc ttttgttccc
     7681 tttagtgagg gttaatttcg agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     7741 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     7801 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     7861 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     7921 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     7981 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     8041 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     8101 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     8161 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     8221 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     8281 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     8341 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     8401 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     8461 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     8521 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     8581 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     8641 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     8701 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     8761 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     8821 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     8881 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     8941 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     9001 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     9061 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     9121 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     9181 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     9241 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     9301 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     9361 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     9421 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     9481 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     9541 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     9601 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     9661 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     9721 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     9781 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     9841 cgcgcacatt tccccgaaaa gtgccac
//
