LOCUS       sequence-111690-        9834 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tol2-based Wnt signal reporter plasmid (expresses NLS-ONL-CP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9834)
  AUTHORS   Takai A, Nakano M, Saito K, Haruno R, Watanabe TM, Ohyanagi T, Jin 
            T, Okada Y, Nagai T
  TITLE     Expanded palette of Nano-lanterns for real-time multicolor 
            luminescence imaging.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):4352-6. doi: 
            10.1073/pnas.1418468112. Epub 2015 Mar 23.
  PUBMED    25831507
REFERENCE   2  (bases 1 to 9834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1418468112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-04-7- 7"; volume: "112"; issue: "14"; pages: "4352-6"
COMMENT     Sequence Label: pT2-7xTcf-NLS-ONL-CP
FEATURES             Location/Qualifiers
     source          1..9834
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     623..642
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(655..666,667..854)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     polyA_signal    2123..2171
                     /note="synthetic polyadenylation signal"
     primer_bind     2346..2370
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2460..2480
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2484..2504
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2508..2528
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2541..3194
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
     CDS             2541..3194
                     /codon_start=1
                     /product="humanized monomeric Kusabira-Orange fluorescent 
                     protein"
                     /label=hmKO
                     /note="mammalian codon-optimized"
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
     CDS             4125..4172
                     /codon_start=1
                     /product="non-ORF yeast peptide conferring 
                     ubiquitin-dependent degradation"
                     /label=hCL1
                     /note="human codon-optimized"
                     /translation="ACKNWFSSLSHFVIHL"
     CDS             4176..4295
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     polyA_signal    4358..4582
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4629..4652)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5026..5045
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5110..5709
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5110..5129)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5606..5626
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    5796..6020
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6048..6064)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6072..6088
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6096..6102,6103..6120,6121..6126))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6141..6162
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    join(7478..7615,7616..7627)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     promoter        complement(7646..7664)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7685..7701)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7709..7725
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7733..7739,7740..7757,7758..7763))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7778..7799
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7916..7933)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8087..8675)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8167..8186)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8846..9637,9638..9706))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9469..9488
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9707..9811)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtaccagagg
      661 tgtaaagtac ttgagtaatt ttacttgatt actgtactta agtattattt ttggggattt
      721 ttactttact tgagtacaat taaaaatcaa tacttttact tttacttaat tacatttttt
      781 tagaaaaaaa agtacttttt actccttaca attttattta cagtcaaaaa gtacttattt
      841 tttggagatc acttgtcgag tagggataac agggtaatga tcccccgggc tgcagtcgcg
      901 acgcgtccta gagggacagc ccccccccaa agcccccagg gatgtaatta cgtccctccc
      961 ccgctagggg gcagcagcga gccgcccggg gctccgctcc ggtccggcgc tccccccgca
     1021 tccccgagcc ggcagcgtgc ggggacagcc cgggcacggg gaaggtggca cgggatcgct
     1081 ttcctctgaa cgcttctcgc tgctctttga gcctgcagac acctgggggg atacggggaa
     1141 aaagctttag gctgaaagag agatttagaa tgacagaatc atagaacggc ctgggttgca
     1201 aaggagcaca gtgctcatcc agatccaacc ccctgctatg tgcagggtca tcaaccagca
     1261 gcccaggctg cccagagcca catccagcct ggccttgaat gcctgcaggg atggggcatc
     1321 cacagcctcc ttgggcaacc tgttcagtgc gtcaccaccc tctgggggaa aaactgcctc
     1381 ctcatatcca acccaaacct cccctgtctc agtgtaaagc cattccccct tgtcctatca
     1441 agggggagtt tgctgtgaca ttgttggtct ggggtgacac atgtttgcca attcagtgca
     1501 tcacggagag gcagatcttg gggataagga agtgcaggac agcatggacg tgggacatgc
     1561 aggtgttgag ggctctggga cactctccaa gtcacagcgt tcagaacagc cttaaggata
     1621 agaagatagg atagaaggac aaagagcaag ttaaaaccca gcatggagag gagcacaaaa
     1681 aggccacaga cactgctggt ccctgtgtct gagcctgcat gtttgatggt gtctggatgc
     1741 aagcagaagg ggtggaagag cttgcctgga gagatacagc tgggtcagta ggactgggac
     1801 aggcagctgg agaattgcca tgtagatgtt catacaatcg tcaaatcatg aaggctggaa
     1861 aagccctcca agatccccaa gaccaacccc aacccaccca ccgtgcccac tggccatgtc
     1921 cctcagtgcc acatccccac agttcttcat cacctccagg gacggtgacc cccccacctc
     1981 cgtgggcagc tgtgccactg cagcaccgct ctttggagaa ggtaaatctt gctaaatcca
     2041 gcccgaccct cccctggcac aacgtaaggc cattatctct catccaactc caggacggag
     2101 tcagtgagga tggggctcta ggaataaaat atctttattt tcattacatc tgtgtgttgg
     2161 ttttttgtgt ggtcgacggt accgagctct tacgcgagat caaagggggt aagatcaaag
     2221 ggggtaagat caaaggggcg cgagatcaaa gggggtaaga tcaaaggggg taagatcaaa
     2281 gggggtaaga tcaaaggggc gcgcccgcgt gctaggcgtg tacggtggga ggcctatata
     2341 agcagagctc gtttagtgaa ccgtcagatc gcatcgattc gaattccagc ttggcattcc
     2401 ggtactgttg gtaaaaagct tggcattccg gtactgttgg taaagccacc atgctggtac
     2461 caaaaaagaa gagaaaggtt gtaccaaaaa agaagagaaa ggttgtacca aaaaagaaga
     2521 gaaaggtttt cgaagggccc atggtgagcg tgatcaagcc cgagatgaag atgaggtact
     2581 acatggacgg ctccgtcaat gggcatgagt tcacaatcga gggtgagggc acaggcagac
     2641 cttacgaggg acatcaggag atgacactgc gcgtcacaat ggccgagggc gggccaatgc
     2701 ctttcgcctt cgacctggtg tcccacgtgt tctgttacgg ccacagagtt tttactaaat
     2761 atccagaaga gatcccagac tatttcaagc aggcctttcc tgagggcctg tcctgggaga
     2821 ggtccctgga gttcgaggac ggcggctccg cctccgtgag cgcccacatc agcctgaggg
     2881 gcaacacctt ctaccacaag tccaagttca ccggcgtgaa cttccccgcc gacggcccca
     2941 tcatgcagaa ccagagcgtg gactgggagc cctccaccga gaagatcacc gccagcgacg
     3001 gcgtgctgaa gggcgacgtg accatgtacc tgaagctgga gggcggcggc aaccacaagt
     3061 gccagatgaa gaccacctac aaggccgcca aggagatcct ggagatgccc ggcgaccact
     3121 acatcggcca caggctggtg aggaagaccg agggcaacat caccgagcag gtggaggacg
     3181 ccgtggccca ctccggtacc aaggtgtacg accccgagca acgcaaacgc atgatcactg
     3241 ggcctcagtg gtgggctcgc tgcaagcaaa tgaacgtgct ggactccttc atcaactact
     3301 atgattccga gaagcacgcc gagaacgccg tgatttttct gcatggtaac gctacctcca
     3361 gctacctgtg gaggcacgtc gtgcctcaca tcgagcccgt ggctagatgc atcatccctg
     3421 atctgatcgg aatgggtaag tccggcaaga gcgggaatgg ctcatatcgc ctcctggatc
     3481 actacaagta cctcaccgct tggttcgagc tgctgaacct tccaaagaaa atcatctttg
     3541 tgggccacga ctgggggagc gctctggcct ttcactacgc ctacgagcac caagacagga
     3601 tcaaggccat cgtccatatg gagagtgtcg tggacgtgat cgagtcctgg atggggtggc
     3661 ctgacatcga ggaggagctg gccctgatca agagcgaaga gggcgagaaa atggtgcttg
     3721 agaataactt cttcgtcgag accctgttgc caagcaagat catgcggaaa ctggagcctg
     3781 aggagttcgc tgcctacctg gagccattca aggagaaggg cgaggttaga cggcctaccc
     3841 tctcctggcc tcgcgagatc cctctcgtta agggaggcaa gcccgacgtc gtccagattg
     3901 tccgcaacta caacgcctac cttcgggcca gcgacgatct gcctaagctg ttcatcgagt
     3961 ccgaccctgg gttcttttcc aacgctattg tcgagggagc taagaagttc cctaacaccg
     4021 agttcgtgaa ggtgaagggc ctccacttcc tccaggagga cgctccagat gaaatgggta
     4081 agtacatcaa gagcttcgtg gagcgcgtgc tgaagaacga gcaggcttgc aagaactggt
     4141 tcagtagctt aagccacttt gtgatccacc ttaacagcca cggcttccct cccgaggtgg
     4201 aggagcaggc cgccggcacc ctgcccatga gctgcgccca ggagagcggc atggatagac
     4261 accctgctgc ttgcgccagc gccaggatca acgtctaatc tagagcggcc gctcgataag
     4321 cttgatcgat gatctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca
     4381 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     4441 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     4501 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     4561 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa cctgcagctt ttcccaaggc
     4621 agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct
     4681 ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc
     4741 ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg
     4801 cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca
     4861 gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct
     4921 ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg
     4981 ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc
     5041 aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgtg
     5101 gccacaacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc
     5161 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc
     5221 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc
     5281 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc
     5341 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag
     5401 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg
     5461 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag
     5521 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc
     5581 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc
     5641 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc
     5701 ggtgcctgac gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc acgaccccat
     5761 gcatcgatga tctagagctc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     5821 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     5881 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     5941 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     6001 ggatgcggtg ggctctatgg ctgcagccca agcttggcgt aatcatggtc atagctgttt
     6061 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     6121 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     6181 cccgctttcc agtcgggaaa cctgtcgtgc cagcggatcc ctagagggac agcccccccc
     6241 caaagccccc agggatgtaa ttacgtccct cccccgctag ggggcagcag cgagccgccc
     6301 ggggctccgc tccggtccgg cgctcccccc gcatccccga gccggcagcg tgcggggaca
     6361 gcccgggcac ggggaaggtg gcacgggatc gctttcctct gaacgcttct cgctgctctt
     6421 tgagcctgca gacacctggg gggatacggg gaaaaagctt taggctgaaa gagagattta
     6481 gaatgacaga atcatagaac ggcctgggtt gcaaaggagc acagtgctca tccagatcca
     6541 accccctgct atgtgcaggg tcatcaacca gcagcccagg ctgcccagag ccacatccag
     6601 cctggccttg aatgcctgca gggatggggc atccacagcc tccttgggca acctgttcag
     6661 tgcgtcacca ccctctgggg gaaaaactgc ctcctcatat ccaacccaaa cctcccctgt
     6721 ctcagtgtaa agccattccc ccttgtccta tcaaggggga gtttgctgtg acattgttgg
     6781 tctggggtga cacatgtttg ccaattcagt gcatcacgga gaggcagatc ttggggataa
     6841 ggaagtgcag gacagcatgg acgtgggaca tgcaggtgtt gagggctctg ggacactctc
     6901 caagtcacag cgttcagaac agccttaagg ataagaagat aggatagaag gacaaagagc
     6961 aagttaaaac ccagcatgga gaggagcaca aaaaggccac agacactgct ggtccctgtg
     7021 tctgagcctg catgtttgat ggtgtctgga tgcaagcaga aggggtggaa gagcttgcct
     7081 ggagagatac agctgggtca gtaggactgg gacaggcagc tggagaattg ccatgtagat
     7141 gttcatacaa tcgtcaaatc atgaaggctg gaaaagccct ccaagatccc caagaccaac
     7201 cccaacccac ccaccgtgcc cactggccat gtccctcagt gccacatccc cacagttctt
     7261 catcacctcc agggacggtg acccccccac ctccgtgggc agctgtgcca ctgcagcacc
     7321 gctctttgga gaaggtaaat cttgctaaat ccagcccgac cctcccctgg cacaacgtaa
     7381 ggccattatc tctcatccaa ctccaggacg gagtcagtga ggatggggct ctagccggac
     7441 cgacgcgtct cgatagggat aacagggtaa ttgatccaat actcaagtac aattttaatg
     7501 gagtactttt ttacttttac tcaagtaaga ttctagccag atacttttac ttttaattga
     7561 gtaaaatttt ccctaagtac ttgtactttc acttgagtaa aatttttgag tactttttac
     7621 acctctggag ctccagcttt tgttcccttt agtgagggtt aatttcgagc ttggcgtaat
     7681 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     7741 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     7801 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     7861 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     7921 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     7981 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     8041 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     8101 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     8161 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     8221 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     8281 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     8341 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     8401 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     8461 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     8521 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     8581 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     8641 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     8701 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     8761 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     8821 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     8881 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     8941 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     9001 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     9061 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     9121 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     9181 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     9241 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     9301 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     9361 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     9421 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     9481 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     9541 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     9601 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     9661 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     9721 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     9781 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccac
//
