LOCUS       sequence-111695-         799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  generating transgenic TRIC flies.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Gao XJ, Riabinina O, Li J, Potter CJ, Clandinin TR, Luo L
  TITLE     A transcriptional reporter of intracellular Ca in Drosophila.
  JOURNAL   Nat Neurosci. 2015 May 11. doi: 10.1038/nn.4016.
  PUBMED    25961791
REFERENCE   2  (bases 1 to 799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2015 May 11. doi: 10.1038/nn.4016."
COMMENT     Sequence Label: pattB-nsyb-MKIIK11A::nlsLexADBDo
FEATURES             Location/Qualifiers
     source          1..799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             137..157
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(167..418,419..775)
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
ORIGIN
        1 atcatcgaca gccgaattca ccatgtttaa tgcgcggcgc aagctagccg gagccatact
       61 tacgacaatg ttggcgacca gaaatttttc ggctagcgga ggaggtggtg gaggtggagg
      121 tggaggtact agtccaccca agaagaagcg aaaagtagaa gatccaatga aggctctcac
      181 ggcccgacaa caggaagttt ttgatttgat acgggatcat atatcccaaa cgggtatgcc
      241 tccgacccgc gcagagatag cacagcgact gggctttcga tcgcctaacg ccgcggagga
      301 gcacttgaag gcactggccc gcaagggtgt cattgaaatc gtgtccggtg cgagccgcgg
      361 aatccggctg ttgcaggagg aggaagaggg cctgccactg gtgggacgcg tggccgctgg
      421 cgagccgctg ctggcccagc aacacataga gggacactat caggtggacc cctccttgtt
      481 taagccaaat gctgatttcc tgttgcgggt gtcgggaatg tccatgaagg acatcggtat
      541 tatggatggt gacctcctcg ccgtccataa gacacaggat gtcaggaacg gccaggtagt
      601 cgttgccagg atagacgatg aggtcactgt gaaacgtctc aagaagcaag gcaataaggt
      661 cgagctgctg ccggagaata gcgagttcaa gccgatcgtg gtggatctgc gacagcagtc
      721 ctttactatc gagggcttgg ccgtgggtgt gatccgcaac ggagattggc tgtaagacgt
      781 ctatcgatac cgtcgacta
//
