LOCUS       sequence-111702-         799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  generating transgenic TRIC flies.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Gao XJ, Riabinina O, Li J, Potter CJ, Clandinin TR, Luo L
  TITLE     A transcriptional reporter of intracellular Ca in Drosophila.
  JOURNAL   Nat Neurosci. 2015 May 11. doi: 10.1038/nn.4016.
  PUBMED    25961791
REFERENCE   2  (bases 1 to 799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2015 May 11. doi: 10.1038/nn.4016."
COMMENT     Sequence Label: pattB-nsyb-MKII::nlsLexADBDo
FEATURES             Location/Qualifiers
     source          1..799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             136..156
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(166..417,418..774)
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
ORIGIN
        1 tcatcgacag ccgaattcac catgtttaat gcgcggcgca agctaaaggg agccatactt
       61 acgacaatgt tggcgaccag aaatttttcg gctagcggag gaggtggtgg aggtggaggt
      121 ggaggtacta gtccacccaa gaagaagcga aaagtagaag atccaatgaa ggctctcacg
      181 gcccgacaac aggaagtttt tgatttgata cgggatcata tatcccaaac gggtatgcct
      241 ccgacccgcg cagagatagc acagcgactg ggctttcgat cgcctaacgc cgcggaggag
      301 cacttgaagg cactggcccg caagggtgtc attgaaatcg tgtccggtgc gagccgcgga
      361 atccggctgt tgcaggagga ggaagagggc ctgccactgg tgggacgcgt ggccgctggc
      421 gagccgctgc tggcccagca acacatagag ggacactatc aggtggaccc ctccttgttt
      481 aagccaaatg ctgatttcct gttgcgggtg tcgggaatgt ccatgaagga catcggtatt
      541 atggatggtg acctcctcgc cgtccataag acacaggatg tcaggaacgg ccaggtagtc
      601 gttgccagga tagacgatga ggtcactgtg aaacgtctca agaagcaagg caataaggtc
      661 gagctgctgc cggagaatag cgagttcaag ccgatcgtgg tggatctgcg acagcagtcc
      721 tttactatcg agggcttggc cgtgggtgtg atccgcaacg gagattggct gtaagacgtc
      781 tatcgatacc gtcgactaa
//
