LOCUS       sequence-111707-        9977 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mouse Oct4 targeting vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9977)
  AUTHORS   Ochiai H, Sugawara T, Yamamoto T
  TITLE     Simultaneous live imaging of the transcription and nuclear position 
            of specific genes.
  JOURNAL   Nucleic Acids Res. 2015 Jun 19. pii: gkv624.
  PUBMED    26092696
REFERENCE   2  (bases 1 to 9977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Jun 19. pii: gkv624."
COMMENT     Sequence Label: pTV-Oct4-TK-HMS
FEATURES             Location/Qualifiers
     source          1..9977
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1956..2009
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     protein_bind    complement(2010..2043)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             2046..3173
                     /codon_start=1
                     /product="herpes simplex virus thymidine kinase"
                     /label=HSV TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MASYPCHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRLEQK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWQVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
                     HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFILDYDQSPAGCRDAL
                     LQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     CDS             3183..3236
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3237..4259
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             3237..4259
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(4260..4293)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      7370..7379
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(7789..7809)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7789..7807)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7828..7844)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7828..7844)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7841..7863)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7852..7868
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7876..7882,7883..7900,7901..7906))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7921..7942
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8059..8076)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8230..8818)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8310..8329)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8989..9780,9781..9849))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9612..9631
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9850..9954)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgtgatctca
      661 cggctcttaa tgtaggcgca agggggtggg gcatcttagg agctgcttct ccacaggtaa
      721 gggaggatta gacgcttgta gcttgaactg tcagaggtgg gggcttgggc tcccttcttg
      781 ctgcctcact cactctgttt gatcggcctt tcaggaaagg tgttcagcca gaccaccatc
      841 tgtcgcttcg aggccttgca gctcagcctt aagaacatgt gtaagctgcg gcccctgctg
      901 gagaagtggg tggaggaagc cgacaacaat gagaaccttc aggaggtgag gagtggcagg
      961 atgtgtgcaa tgtctgccag gcacagtccc ttctgctgct tccattcctg gcttgaaact
     1021 cctccctctc caaccggagc tcgcaggaga agttctgtgt ccttattctg ctgctatgaa
     1081 ttggaatcca gagccttaac atttgctaat caatcaggct ctctccttct gagtcaccct
     1141 ctgcccccac cagcctgaca atggtccctc cccagaaccc cgtctagtgc tggtgaaggc
     1201 tcagacctag gtctaccagc cccttccaga gcccctttca gtaacccctg gctctggggc
     1261 cacatccaat caatgctccc ttagcacaat cccttagcgg tttgttcttc agtcccatct
     1321 caaggtgggg ctgttgccaa gtcaaatact aaagttgctc ttgtcgcccc catcttcccc
     1381 tgcccagata tgcaaatcgg agaccctggt gcaggcccgg aagagaaagc gaactagcat
     1441 tgagaaccgt gtgaggtgga gtctggagac catgtttctg aagtgcccga agccctccct
     1501 acagcagatc actcacatcg ccaatcagct tgggctagag aaggatgtga gtgccaagat
     1561 cctgccctgt ggtacctgga tgtttccctg ttcccattcc ccaccccccc caccccccca
     1621 cccccaccgc cgccaccgct gactgcagca tcccagagct tatgatctga tgtccatctc
     1681 tgtgcccatc ctaggtggtt cgagtatggt tctgtaaccg gcgccagaag ggcaaaagat
     1741 caagtattga gtattcccaa cgagaagagt acgaggctac cggtacccca tttccaggtg
     1801 gagctgtatc tttcccactg cctcctggtc cccactttgg caccccaggc tatggaagcc
     1861 cccacttcac cacactctac tcagtccctt ttcctgaggg cgaggccttt ccctctgttc
     1921 ccgtcactgc tctgggctct cccatgcatt caaacgaggg aagaggtagc ctgctaactt
     1981 gcggggacgt agaggaaaat cctggtccca taacttcgta tagcatacat tatacgaagt
     2041 tattgatggc ttcgtacccc tgccatcaac acgcgtctgc gttcgaccag gctgcgcgtt
     2101 ctcgcggcca tagcaaccga cgtacggcgt tgcgccctcg ccggcagcaa gaagccacgg
     2161 aagtccgcct ggagcagaaa atgcccacgc tactgcgggt ttatatagac ggtcctcacg
     2221 ggatggggaa aaccaccacc acgcaactgc tggtggccct gggttcgcgc gacgatatcg
     2281 tctacgtacc cgagccgatg acttactggc aggtgctggg ggcttccgag acaatcgcga
     2341 acatctacac cacacaacac cgcctcgacc agggtgagat atcggccggg gacgcggcgg
     2401 tggtaatgac aagcgcccag ataacaatgg gcatgcctta tgccgtgacc gacgccgttc
     2461 tggctcctca tatcgggggg gaggctggga gctcacatgc cccgcccccg gccctcaccc
     2521 tcatcttcga ccgccatccc atcgccgccc tcctgtgcta cccggccgcg cgatacctta
     2581 tgggcagcat gaccccccag gccgtgctgg cgttcgtggc cctcatcccg ccgaccttgc
     2641 ccggcacaaa catcgtgttg ggggcccttc cggaggacag acacatcgac cgcctggcca
     2701 aacgccagcg ccccggcgag cggcttgacc tggctatgct ggccgcgatt cgccgcgttt
     2761 acgggctgct tgccaatacg gtgcggtatc tgcagggcgg cgggtcgtgg cgggaggatt
     2821 ggggacagct ttcggggacg gccgtgccgc cccagggtgc cgagccccag agcaacgcgg
     2881 gcccacgacc ccatatcggg gacacgttat ttaccctgtt tcgggccccc gagttgctgg
     2941 cccccaacgg cgacctgtac aacgtgtttg cctgggcctt ggacgtcttg gccaaacgcc
     3001 tccgtcccat gcacgtcttt atcctggatt acgaccaatc gcccgccggc tgccgggacg
     3061 ccctgctgca acttacctcc gggatggtcc agacccacgt caccaccccc ggctccatac
     3121 cgacgatctg cgacctggcg cgcacgtttg cccgggagat gggggaggct aactctcgag
     3181 ctgagggaag aggtagcctg ctaacttgcg gggacgtaga ggaaaatcct ggtcccatga
     3241 aaaagcctga actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg
     3301 tctccgacct gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag
     3361 gagggcgtgg atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt
     3421 atgtttatcg gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg
     3481 aattcagcga gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag
     3541 acctgcctga aaccgaactg cccgctgttc tgcagccggt cgcggaggcc atggatgcga
     3601 tcgctgcggc cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg
     3661 gtcaatacac tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact
     3721 ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga
     3781 tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca
     3841 acaatgtcct gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt
     3901 tcggggattc ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta
     3961 tggagcagca gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc
     4021 tccgggcgta tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca
     4081 atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg
     4141 ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg
     4201 tagaagtact cgccgatagt ggaaaccgac gccccagcac tcgtccgagg gcaaaggaaa
     4261 taacttcgta tagcatacat tatacgaagt tatgaggatc cattccagcg gccgcgaatt
     4321 cgcccttcgc gggatcctac ggtacttatt gccaagaaag cacgagcatc agccgtgcct
     4381 ccaggtcgaa tcttcaaacg acgacgatca cgcgtcgctc cagtattcca gggttcatca
     4441 gatcctacgg tacttattgc caagaaagca cgagcatcag ccgtgcctcc aggtcgaatc
     4501 ttcaaacgac gacgatcacg cgtcgctcca gtattccagg gttcatcaga tcctacggta
     4561 cttattgcca agaaagcacg agcatcagcc gtgcctccag gtcgaatctt caaacgacga
     4621 cgatcacgcg tcgctccagt attccagggt tcatcagatc ctacggtact tattgccaag
     4681 aaagcacgag catcagccgt gcctccaggt cgaatcttca aacgacgacg atcacgcgtc
     4741 gctccagtat tccagggttc atcagatcct acggtactta ttgccaagaa agcacgagca
     4801 tcagccgtgc ctccaggtcg aatcttcaaa cgacgacgat cacgcgtcgc tccagtattc
     4861 cagggttcat cagatcctac ggtacttatt gccaagaaag cacgagcatc agccgtgcct
     4921 ccaggtcgaa tcttcaaacg acgacgatca cgcgtcgctc cagtattcca gggttcatca
     4981 gatcctacgg tacttattgc caagaaagca cgagcatcag ccgtgcctcc aggtcgaatc
     5041 ttcaaacgac gacgatcacg cgtcgctcca gtattccagg gttcatcaga tcctacggta
     5101 cttattgcca agaaagcacg agcatcagcc gtgcctccag gtcgaatctt caaacgacga
     5161 cgatcacgcg tcgctccagt attccagggt tcatcagatc ctacggtact tattgccaag
     5221 aaagcacgag catcagccgt gcctccaggt cgaatcttca aacgacgacg atcacgcgtc
     5281 gctccagtat tccagggttc atcagatcct acggtactta ttgccaagaa agcacgagca
     5341 tcagccgtgc ctccaggtcg aatcttcaaa cgacgacgat cacgcgtcgc tccagtattc
     5401 cagggttcat cagatcctac ggtacttatt gccaagaaag cacgagcatc agccgtgcct
     5461 ccaggtcgaa tcttcaaacg acgacgatca cgcgtcgctc cagtattcca gggttcatca
     5521 gatcctacgg tacttattgc caagaaagca cgagcatcag ccgtgcctcc aggtcgaatc
     5581 ttcaaacgac gacgatcacg cgtcgctcca gtattccagg gttcatcaga tctagtgggc
     5641 accagccctc cctggggatg ctgtgagcca aggcaaggga ggtagacaag agaacctgga
     5701 gctttggggt taaattcttt tactgaggag ggattaaaag cacaacaggg gtggggggtg
     5761 ggatggggaa agaagctcag tgatgctgtt gatcaggagc ctggcctgtc tgtcactcat
     5821 cattttgttc ttaaataaag actgggacac acagtagata gctgaatttt gttttccttc
     5881 agttcctaga gagcctgcgg ttggagaaag ccagtaatgg attctcaaac cccaggtgat
     5941 cttcaaaaca ggcgccattg aaaccattgg agttccacaa aatgcccagg gatagttggg
     6001 gttggagccc aacctataga ggaaggcatt gcatattcgc catcctagag gcggtaagtc
     6061 tctgctagct gatggacatc acctcatagc cattgtctgg cagccgcctt ctttcctctt
     6121 gtcactctgg gagttctggt gggcttatac tttaaaaaaa agagtttttt tgggggggtt
     6181 aagatttatt ttatttatat gggtacactg tagctgtctt ctagacacac cagaagaggg
     6241 catgggatcc cattacagat ggttgtgagc caccatgtgg ttgctgggaa ttgaactcag
     6301 gacctctgga agagcagtca gtgctcttaa ccgctgagcc atctctccag ccctcaaact
     6361 cttttttttc ttttccttca agatgagttc tgtgtagtcc tggcggacca ggttggcctc
     6421 agatcagcct gcctctgcct ccgcagtgct gagattaaag gcccgtgcca ctctaggcta
     6481 aattgttatg cttctattct agctgatgac cacctttttt gggcgtagta gtgctgggag
     6541 tagggtctgt acacatgtct acaatgccag aataggtcaa aggctttaga tctcaaggaa
     6601 ctggatttat agagagttgg gagcagccat gtaggttctg agaaccaaac ctgggtcctc
     6661 tgcaagaaga gccattggct ttttgttttt gtttgttttg agacatttct cggtgtagcc
     6721 ctggctatct ggaactctgt aggccaggct gtccctgaac tcagatccag tctatccatc
     6781 cctgccttcc aagagctggg attaaggtca tgtaccacca caggccagct agccatagct
     6841 cctaactgct gaaccattta tttatttatt tattttattt ttttggtttt tcgagacagg
     6901 gtttctctgt atagctctgg atgtcctgga actcactttg taaaccagtc tggcctcgaa
     6961 ctcagaaatc tgcttgcctc tgcctcccca gtgagtgctg ggatcaaatg cgtgcgccac
     7021 cactcccagc ttaagtcttt attttttaaa atgttattta ttttggggct ggtgagatgg
     7081 ctcagtggtt aagagcactg actgctctgc cagaggtccc gagttcaaat cccagcaact
     7141 acatggtggc tcacaaccat ctgtaatgag atctgacgcc ctcttctggt atgtctgaag
     7201 acaactacag tgtacttaca tatatatata ataaataaat taaaaaaaaa aaagagagga
     7261 ggagccaagc agctccttta gaaaaaaaaa aagttattta ttttatgtat ttgagctgtc
     7321 ttcagacaca ccagaagagg gcaatggatc ccattacaga tggttgtgag ccaccatggt
     7381 tgatgggaat tgaactctgg acctctggca gagcagtcag tgctcttaac cgctgagcca
     7441 tctctccagc ccccaaagcc aagtcttaaa gcatttttgc tgctgaatgt cagccctacc
     7501 agatctctgc ctccctcccc tcccctcccc ccagtatctc atgaagacca agctggcctg
     7561 gacacagtaa gtatgtgcat atttatgtgt gaagattgtc acaatgtgag gaaaaaagtt
     7621 ggttccctcc atggtgtggg tcctgtgggt caagtccagg tcgctaggct tggcagcaag
     7681 tgccttgact catagtcttc tcctgcccaa ctccatgctt ggtttccacg agcccctgtg
     7741 ctatggagaa ttccatctcc aggcctcacc aaatctcagc ttttgttccc tttagtgagg
     7801 gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     7861 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     7921 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     7981 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     8041 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     8101 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     8161 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     8221 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     8281 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     8341 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     8401 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     8461 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     8521 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     8581 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     8641 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     8701 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     8761 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     8821 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     8881 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     8941 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     9001 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     9061 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     9121 gataccgcgg ctgccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     9181 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     9241 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     9301 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     9361 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     9421 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     9481 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     9541 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     9601 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     9661 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     9721 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     9781 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     9841 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     9901 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     9961 tccccgaaaa gtgccac
//
