LOCUS       sequence-111708-         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Flag-tagged biotinylation enzyme for co-expression with 
            bio-proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Bushell KM, Sollner C, Schuster-Boeckler B, Bateman A, Wright GJ
  TITLE     Large-scale screening for novel low-affinity extracellular protein 
            interactions.
  JOURNAL   Genome Res. 2008 Apr;18(4):622-30. Epub 2008 Feb 22.
  PUBMED    18296487
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Res."; date: "2008-04"; volume: "18(4)"; pages: "622-30. Epub 2008 
            Feb 22"
COMMENT     Sequence Label: secreted BirA-Flag
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(46..69)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ataaatttcc tttattagcc agaggtcgag gtcgggggat cctcacttgt catcatcgtc
       61 cttgtagtcg gggcccttct cggcgcttct caggctgatc tcgcctccca tccagggctt
      121 gatgatgccg tcctgctcca gcagcagggc gccctgcttg tcgatgcctc tgctgatgcc
      181 gaagatctcc ttgtcgccga tgatcagctt cacgggtctg ttgatgaagt tgtccagctt
      241 ctcccatctg ctcaggtagg gagccaggcc ctcctgctcg aacagctcca gtgcggctct
      301 cagctctctg atcagcatgg ctgccagggt gtttctgtcc aggttgatgc cggcctcctg
      361 cagggtgatc cagccctggt tcaccacgct ttcctccact cttctcatgg ccatgttgat
      421 gccggcgccg atcacgatct gtgcggcgtc gccggtcttg ccggtcagct ccaccaggat
      481 gccggccagc tttctgtcct gcaggtacag gtcgttgggc cacttcactc tcaccttgtc
      541 ggcgcccagc tttctcagca cctcggccat cacgatgccg atcaccaggc tcaggccgat
      601 tgcggctgcg gggccctgct ccagtctcca gaacatgctc aggtacaggt tggcgccaaa
      661 ggggctgaac cactttctgc ctcttctgcc tctgccggcc tgctggtact cggcgatgca
      721 ggcgtcgccg ctcttcagct cgccgattct gtccagcagg tactggttgg tgctgtcgat
      781 cacgggcagc acggccacgc ttccgccgtc cagctggcc
//
