LOCUS       sequence-111712-        1113 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Co-expression of human codon-optimized Cas9 nuclease and GFP, 
            plasmid optimized for expression in human pluripotent stem cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Ding Q, Regan SN, Xia Y, Oostrom LA, Cowan CA, Musunuru K
  TITLE     Enhanced efficiency of human pluripotent stem cell genome editing 
            through replacing TALENs with CRISPRs.
  JOURNAL   Cell Stem Cell. 2013 Apr 4;12(4):393-4. doi: 
            10.1016/j.stem.2013.03.006.
  PUBMED    23561441
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2013.03"; journalName: "Cell Stem Cell"; date: 
            "2013-04-4- 4"; volume: "12"; issue: "4"; pages: "393-4"
COMMENT     Sequence Label: pCas9_GFP
FEATURES             Location/Qualifiers
     source          1..1113
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(33..86)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(102..122)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ccggtgaaca gctcctcgcc cttgctcacc attgggccgg gattctcctc cacgtcaccg
       61 catgttagaa gacttcctct gccctctccg ccgccggacc tcaccttcct cttcttcttg
      121 gggtcagccc tgctgtctcc accgagctga gagaggtcga ttcttgtttc atagagcccc
      181 gtaattgact gatgaatcag tgtggcgtcc aggacctcct ttgtagaggt gtaccgcttt
      241 ctgtctatgg tggtgtcgaa gtacttgaag gctgcaggcg cgcccaagtt ggtcagagta
      301 aacaagtgga taatgttttc tgcctgctcc ctgatgggct tatccctgtg cttattgtaa
      361 gcagaaagca ccttatcgag gttagcgtcg gcgaggatca ctcttttgga gaattcgctt
      421 atttgctcga tgatctcatc aaggtagtgt ttgtgttgtt ccacgaacag ctgcttctgc
      481 tcattatctt cgggagaccc tttgagcttt tcatagtggc tggccagata caagaaatta
      541 acgtatttag agggcagtgc cagctcgtta cctttctgca gctcgcccgc actagcgagc
      601 attcgtttcc ggccgttttc aagctcaaag agagagtact tgggaagctt aatgatgagg
      661 tcttttttga cctctttata tcctttcgcc tcgagaaagt cgatggggtt tttttcgaag
      721 cttgatcgct ccatgattgt gatgcccagc agttccttga cgcttttgag ttttttagac
      781 ttccctttct ccactttggc cacaaccagt acactgtaag cgactgtagg agaatcgaat
      841 ccgccgtatt tcttggggtc ccaatctttt ttgcgtgcga tcagcttgtc gctgttcctt
      901 ttcgggagga tactttcctt ggagaagcct ccggtctgta cttcggtctt tttaacgatg
      961 ttcacctgcg gcatggacag gaccttccgg actgtcgcga aatccctacc cttgtcccac
     1021 acgatttctc ctgtttctcc gtttgtttcg ataagtggtc gcttccgaat ctctccattg
     1081 gccagtgtaa tctcggtctt gaaaaaattc ata
//
