LOCUS       sequence-111713-         954 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For expression and purification of a His-TAT-NLS-Cre fusion; HIV-TAT
            promotes cellular uptake of recombinant Cre into mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Peitz M, Pfannkuche K, Rajewsky K, Edenhofer F
  TITLE     Ability of the hydrophobic FGF and basic TAT peptides to promote 
            cellular uptake of recombinant Cre recombinase: a tool for efficient
            genetic engineering of mammalian genomes.
  JOURNAL   Proc Natl Acad Sci U S A. 2002 Apr 2. 99(7):4489-94.
  PUBMED    11904364
REFERENCE   2  (bases 1 to 954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2002 Apr 2. 99(7):4489-94."
COMMENT     Sequence Label: pTriEx-HTNC
FEATURES             Location/Qualifiers
     source          1..954
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     90..109
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(199..222)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 aaaattccaa cacactattg caatgaaaat aaatttcctt tattagccag aagtcagatg
       61 ctcaaggggc ttcatgatgt ccccataatt tttggcagag ggaaaaagat cgatctcagt
      121 ggtatttgtg agccagggca ttggccacac cagccaccac cttctgatag gcagcctgca
      181 cctgaggtta atcacttagt gatggtgatg gtgatggtgg tgctcgagct aatcgccatc
      241 ttccagcagg cgcaccattg cccctgtttc actatccagg ttacggatat agttcatgac
      301 aatatttaca ttggtccagc caccagcttg catgatctcc ggtattgaaa ctccagcgcg
      361 ggccatatct cgcgcggctc cgacacgggc actgtgtcca gaccaggcca ggtatctctg
      421 accagagtca tccttagcgc cgtaaatcaa tcgatgagtt gcttcaaaaa tcccttccag
      481 ggcgcgagtt gatagctggc tggtggcaga tggcgcggca acaccatttt ttctgacccg
      541 gcaaaacagg tagttattcg gatcatcagc tacaccagag acggaaatcc atcgctcgac
      601 cagtttagtt acccccaggc taagtgcctt ctctacacct gcggtgctaa ccagcgtttt
      661 cgttctgcca atatggatta acattctccc accgtcagta cgtgagatat ctttaaccct
      721 gatcctggca atttcggcta tacgtaacag ggtgttataa gcaatcccca gaaatgccag
      781 attacgtata tcctggcagc gatcgctatt ttccatgagt gaacgaacct ggtcgaaatc
      841 agtgcgttcg aacgctagag cctgttttgc acgttcaccg gcatcaacgt tttcttttcg
      901 gatccgccgc ataaccagtg aaacagcatt gctgtcactt ggtcgtggca gccc
//
