LOCUS       sequence-111720-         932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  harbours marker genes LEU2 and URA3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Mulleder M, Campbell K, Matsarskaia O, Eckerstorfer F, Ralser M
  TITLE     Saccharomyces cerevisiae single-copy plasmids for auxotrophy 
            compensation, multiple marker selection, and for designing 
            metabolically cooperating communities.
  JOURNAL   F1000Res. 2016 Sep 20;5:2351. eCollection 2016.
  PUBMED    27830062
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "F1000Res.";
            date: "2016-09-20"; volume: "5"; pages: "2351. eCollection 2016"
COMMENT     Sequence Label: pLU
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     449..471
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(509..527)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ccctccatgg aaaaatcagt caagatatcc acatgtgttt ttagtaaaca aattttggga
       61 cctaatgctt caactaactc cagtaattcc ttggtggtac gaacatccaa tgaagcacac
      121 aagtttgttt gcttttcgtg catgatatta aatagcttgg cagcaacagg actaggatga
      181 gtagcagcac gttccttata tgtagctttc gacatgattt atcttcgttt cctgcaggtt
      241 tttgttctgt gcagttgggt taagaatact gggcaatttc atgtttcttc aacactacat
      301 atgcgtatat ataccaatct aagtctgtgc tccttccttc gttcttcctt ctgttcggag
      361 attaccgaat cgccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
      421 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
      481 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
      541 gtcatgataa taatggtttc ttagggtcct tttcatcacg tgctataaaa ataattataa
      601 tttaaatttt ttaatataaa tatataaatt aaaaatagaa agtaaaaaaa gaaattaaag
      661 aaaaaatagt ttttgttttc cgaagatgta aaagactcta gggggatcgc caacaaatac
      721 taccttttat cttgctcttc ctgctctcag gtattaatgc cgaattgttt catcttgtct
      781 gtgtagaaga ccacacacga aaatcctgtg attttacatt ttacttatcg ttaatcgaat
      841 gtatatctat ttaatctgct tttcttgtct aataaatata tatgtaaagt acgctttttg
      901 ttgaaatttt ttaaaccttt gtttattttt tt
//
