LOCUS       sequence-111721-        1055 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  harbours marker genes LEU2 and URA3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Mulleder M, Campbell K, Matsarskaia O, Eckerstorfer F, Ralser M
  TITLE     Saccharomyces cerevisiae single-copy plasmids for auxotrophy 
            compensation, multiple marker selection, and for designing 
            metabolically cooperating communities.
  JOURNAL   F1000Res. 2016 Sep 20;5:2351. eCollection 2016.
  PUBMED    27830062
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "F1000Res.";
            date: "2016-09-20"; volume: "5"; pages: "2351. eCollection 2016"
COMMENT     Sequence Label: pLU
FEATURES             Location/Qualifiers
     source          1..1055
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(223..630)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
ORIGIN
        1 taacaaaacg gcatcagcct tcttggaggc ttccagcgcc tcatctggaa gtggaacacc
       61 tgtagcatcg atagcagcac caccaattaa atgattttcg aaatcgaact tgacattgga
      121 acgaacatca gaaatagctt taagaacctt aatggcttcg gctgtgattt cttgaccaac
      181 gtggtcacct ggcaaaacga cgatcttctt aggggcagac attagaatgg tatatccttg
      241 aaatatatat atatatattg ctgaaatgta aaaggtaaga aaagttagaa agtaagacga
      301 ttgctaacca cctattggaa aaaacaatag gtccttaaat aatattgtca acttcaagta
      361 ttgtgatgca agcatttagt catgaacgct tctctattct atatgaaaag ccggttccgg
      421 cgctctcacc tttccttttt ctcccaattt ttcagttgaa aaaggtatat gcgtcaggcg
      481 acctctgaaa ttaacaaaaa atttccagtc atcgaatttg attctgtgcg atagcgcccc
      541 tgtgtgttct cgttatgttg aggaaaaaaa taatggttgc taagagattc gaactcttgc
      601 atcttacgat acctgagtat tcccacagtt aactgcggtc aagatatttc ttgaatcagg
      661 cgccttagac cgctcggcca aacaaccaat tacttgttga gaaatagagt ataattatcc
      721 tataaatata acgtttttga acacacatga acaaggaagt acaggacaat tgattttgaa
      781 gagaatgtgg attttgatgt aattgttggg attccatttt taataaggca ataatattag
      841 gtatgtagat atactagaag ttctcctcga tggcgcgcct gatgcggtat tttctcctta
      901 cgcatctgtg cggtatttca caccgcatag ggtaataact gatataatta aattgaagct
      961 ctaatttgtg agtttagtat acatgcattt acttataata cagtttttta gttttgctgg
     1021 ccgcatcttc tcaaatatgc ttcccagcct gcttt
//
