LOCUS       sequence-111731-         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FRET-based biosensor for monitoring Akt activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Gao X, Zhang J
  TITLE     Spatiotemporal analysis of differential Akt regulation in plasma 
            membrane microdomains.
  JOURNAL   Mol Biol Cell. 2008 Oct;19(10):4366-73. doi: 
            10.1091/mbc.E08-05-0449. Epub 2008 Aug 13.
  PUBMED    18701703
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E08-05-0449"; journalName: "Mol Biol Cell"; date: 
            "2008-10"; volume: "19"; issue: "10"; pages: "4366-73"
COMMENT     Sequence Label: pcDNA3-AktAR
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(71..805)
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
     primer_bind     264..283
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     523..544
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(650..671)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ctatagaata gggccctcta gatgcatgct cgagcggccg ccagtgtgat ggatatctgc
       61 agaattctta ctcgatgttg tggcggatct tgaagttggc cttgatgccg ttcttctgct
      121 tgtcggcggt gatatagacg ttgtggctgt tgtagttgta ctccagcttg tgccccagga
      181 tgttgccgtc ctccttgaag tcgatgccct tcagctcgat gcggttcacc agggtgtcgc
      241 cctcgaactt cacctcggcg cgggtcttgt agttgccgtc gtccttgaag aagatggtgc
      301 gctcctggac gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt
      361 cggggtagcg ggcgaagcac tgaaggccgt agcccagggt ggtcacgagg gtgggccagg
      421 gcacgggcag cttgccggtg gtgcagatca gcttcagggt cagcttgccg taggtggcat
      481 cgccctcgcc ctcgccggac acgctgaact tgtggccgtt tacgtcgccg tccagctcga
      541 ccaggatggg caccaccccg gtgaacagct cctcgccctt gctcaccata gatccaccgg
      601 tacctccctt gtacagctcg tccatgccga gagtgatccc ggcggcggtc acgaactcca
      661 gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgct cagcttggac tggtagctca
      721 ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat gggggtgttc tgctggtagt
      781 ggtcggcgag ctgcacgccg cccatgagct cgctgccgcc ggtacctcca aactccggcc
      841 tggggtccgg ccaggtgcac gagcgcggac ga
//
