LOCUS       sequence-111740-        1052 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  harbours marker genes URA3 and MET17.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Mulleder M, Campbell K, Matsarskaia O, Eckerstorfer F, Ralser M
  TITLE     Saccharomyces cerevisiae single-copy plasmids for auxotrophy 
            compensation, multiple marker selection, and for designing 
            metabolically cooperating communities.
  JOURNAL   F1000Res. 2016 Sep 20;5:2351. eCollection 2016.
  PUBMED    27830062
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "F1000Res.";
            date: "2016-09-20"; volume: "5"; pages: "2351. eCollection 2016"
COMMENT     Sequence Label: pUM
FEATURES             Location/Qualifiers
     source          1..1052
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(23..41)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    89..592
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        622..726
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(945..964)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
       61 ataataatgg tttcttaggg tccttttcat cacgtgctat aaaaataatt ataatttaaa
      121 ttttttaata taaatatata aattaaaaat agaaagtaaa aaaagaaatt aaagaaaaaa
      181 tagtttttgt tttccgaaga tgtaaaagac tctaggggga tcgccaacaa atactacctt
      241 ttatcttgct cttcctgctc tcaggtatta atgccgaatt gtttcatctt gtctgtgtag
      301 aagaccacac acgaaaatcc tgtgatttta cattttactt atcgttaatc gaatgtatat
      361 ctatttaatc tgcttttctt gtctaataaa tatatatgta aagtacgctt tttgttgaaa
      421 ttttttaaac ctttgtttat ttttttttct tcattccgta actcttctac cttctttatt
      481 tactttctaa aatccaaata caaaacataa aaataaataa atacagagta aattcccaaa
      541 ttattccatc attaaaagat acgaggcgcg tgtaagttac aggcaagcga tccgtcaggt
      601 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
      661 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
      721 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
      781 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
      841 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
      901 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
      961 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     1021 gacttggttg agtactcacc agtcacagaa aa
//
