LOCUS       sequence-111742-         592 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To express Oct4, Klf4, Sox2, c-Myc and hairpin RNA p53. A single 
            plasmid reprogramming system using a mini-intronic plasmid (MIP)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Diecke S, Lu J, Lee J, Termglinchan V, Kooreman NG, Burridge PW, 
            Ebert AD, Churko JM, Sharma A, Kay MA, Wu JC
  TITLE     Novel codon-optimized mini-intronic plasmid for efficient, 
            inexpensive, and xeno-free induction of pluripotency.
  JOURNAL   Sci Rep. 2015 Jan 28;5:8081. doi: 10.1038/srep08081.
  PUBMED    25628230
REFERENCE   2  (bases 1 to 592)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-01-28"; volume: "5:8081. doi"; pages: " 
            10.1038/srep08081"
COMMENT     Sequence Label: MIP 247 CoMiP 4in1 with shRNA p53
FEATURES             Location/Qualifiers
     source          1..592
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             251..280
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(545..562)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 gttggacagt gtcagagtcc tgagacagat cagcaacaac cgaaaatgca ccagccccag
       61 gtcctcggac accgaggaga atgtcaagag gcgaacacac aacgtcttgg agcgccagag
      121 gaggaacgag ctaaaacgga gcttttttgc cctgcgtgac cagatcccgg agttggaaaa
      181 caatgaaaag gcccccaagg tagttatcct taaaaaagcc acagcataca tcctgtccgt
      241 ccaagcagag gagcaaaagc tcatttctga agaggacttg ttgcggaaac gacgagaaca
      301 gttgaaacac aaacttgaac agctacggaa ctcttgtgcg taagtcgacg aattcggtac
      361 cgcgggcccg ggatccgccc ctctccctcc ccccccccta acgttactgg ccgaagccgc
      421 ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt
      481 ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt
      541 tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc ag
//
