LOCUS       sequence-111743-        1045 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mCherry fused between Cry2 and gamma 9 to study cell migration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   O'Neill PR, Gautam N
  TITLE     Subcellular optogenetic inhibition of G proteins generates signaling
            gradients and cell migration.
  JOURNAL   Mol Biol Cell. 2014 Aug 1;25(15):2305-14. doi: 
            10.1091/mbc.E14-04-0870. Epub 2014 Jun 11.
  PUBMED    24920824
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E14-04-0870"; journalName: "Mol Biol Cell"; date: 
            "2014-08-1- 1"; volume: "25"; issue: "15"; pages: "2305-14"
COMMENT     Sequence Label: Cry2 mcherry Gamma 9 in pcDNA3.1
FEATURES             Location/Qualifiers
     source          1..1045
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(249..266)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(551..570)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     820..838
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 actcgagcgg ccgccactgg tgctggatat ctgcagaatt ctcagcttat tatacaaccg
       61 cctttttcct tgaagggatt cttgtcctca gggatgcctt tgagaaaagg atcgtttcct
      121 gcttgggcct ccacgtactc cttgatttcc tttcccgctt tggaaatcgg aattcttgtg
      181 tttttcactt ctttcttcag ctgctccacc tccatcttca acaggtcctt ctcgctgaga
      241 tcctgggcgt gatggtgatg gtgatgcatg gtacccttgt acagctcgtc catgccgccg
      301 gtggagtggc ggccctcggc gcgttcgtac tgttccacga tggtgtagtc ctcgttgtgg
      361 gaggtgatgt ccaacttgat gttgacgttg taggcgccgg gcagctgcac gggcttcttg
      421 gccttgtagg tggtcttgac ctcagcgtcg tagtggccgc cgtccttcag cttcagcctc
      481 tgcttgatct cgcccttcag ggcgccgtcc tcggggtaca tccgctcgga ggaggcctcc
      541 cagcccatgg tcttcttctg cattacgggg ccgtcggagg ggaagttggt gccgcgcagc
      601 ttcaccttgt agatgaactc gccgtcctgc agggaggagt cctgggtcac ggtcaccacg
      661 ccgccgtcct cgaagttcat cacgcgctcc cacttgaagc cctcggggaa ggacagcttc
      721 aagtagtcgg ggatgtcggc ggggtgcttc acgtaggcct tggagccgta catgaactga
      781 ggggacagga tgtcccaggc gaagggcagg gggccaccct tggtcacctt cagcttggcg
      841 gtctgggtgc cctcgtaggg gcggccctcg ccctcgccct cgatctcgaa ctcgtggccg
      901 ttcacggagc cctccatgtg caccttgaag cgcatgaact ccttgatgat ggccatgtta
      961 tcctcctcgc cttgctcacc ataagctggg ctgctgctcg aacatgatct gtgcttcacg
     1021 ggttcttgaa atagctttac tagta
//
