LOCUS       sequence-111744-         700 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To express Oct4, Klf4, Sox2, c-Myc and hairpin RNA p53. A single 
            plasmid reprogramming system using a mini-intronic plasmid (MIP)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Diecke S, Lu J, Lee J, Termglinchan V, Kooreman NG, Burridge PW, 
            Ebert AD, Churko JM, Sharma A, Kay MA, Wu JC
  TITLE     Novel codon-optimized mini-intronic plasmid for efficient, 
            inexpensive, and xeno-free induction of pluripotency.
  JOURNAL   Sci Rep. 2015 Jan 28;5:8081. doi: 10.1038/srep08081.
  PUBMED    25628230
REFERENCE   2  (bases 1 to 700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-01-28"; volume: "5:8081. doi"; pages: " 
            10.1038/srep08081"
COMMENT     Sequence Label: MIP 247 CoMiP 4in1 with shRNA p53
FEATURES             Location/Qualifiers
     source          1..700
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(393..452)
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
ORIGIN
        1 tggatgatga tgtttttgat gaaggtctcg tcgtccgggt cgcagatgaa actctggttc
       61 accatgtctc ctcccagcag ctcggtcacc atctccagct ggtcggccgt ggagaagctc
      121 ccgccaccgc cgtcgttgtc tccccgaagg gagaagggtg tgaccgcaac gtaggagggc
      181 gagcagagcc cggagcggcg gctaggggac aggggcgggg tgggcagcag ctcgaatttc
      241 ttccagatat cctcgctggg cgccgggggc tgcagctcgc tctgctgctg ctggtagaag
      301 ttctcctcct cgtcgcagta gaaatacggc tgcaccgagt cgtagtcgag gtcatagttc
      361 ctgttggtga agctaacgtt gaggggcatg gagggtccag ggttggactc cacgtctcca
      421 gccagcttca acagggcgta gtttgtgcac tgacgcgtca tgtggctcag gggcagggtg
      481 ccattgatgg cggtgccagg cacagggccg ctctggtagt gctggctcat gtgcagtctg
      541 ctaggggcgg caggttcagg cacttcggcg cctggcaggt acatgctgat catgtcccgc
      601 aggtcgccgg cctgacaagg ggctctgctg tgggagctgc tggtgaccac aggagggctg
      661 ctgctggcct cgctcttgac cacgctgccc atgctgccca
//
