LOCUS       sequence-111746-         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mCherry fused between Cry2 and gamma 9 to study cell migration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  AUTHORS   O'Neill PR, Gautam N
  TITLE     Subcellular optogenetic inhibition of G proteins generates signaling
            gradients and cell migration.
  JOURNAL   Mol Biol Cell. 2014 Aug 1;25(15):2305-14. doi: 
            10.1091/mbc.E14-04-0870. Epub 2014 Jun 11.
  PUBMED    24920824
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E14-04-0870"; journalName: "Mol Biol Cell"; date: 
            "2014-08-1- 1"; volume: "25"; issue: "15"; pages: "2305-14"
COMMENT     Sequence Label: Cry2 mcherry Gamma 9 in pcDNA3.1
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 agctctctgg ctactagaga acccactgct tactggctta tcgaaattaa tacgactcac
       61 tatagggaga cccaagctgg ctagcgctac cggactcaga tctcgaggcc accatgaaga
      121 tggacaaaaa gactatagtt tggtttagaa gagacctaag gattgaggat aatcctgcat
      181 tagcagcagc tgctcacgaa ggatctgttt ttcctgtctt catttggtgt cctgaagaag
      241 aaggacagtt ttatcctgga agagcttcaa gatggtggat gaaacaatca cttgctcact
      301 tatctcaatc cttgaaggct cttggatctg acctcacttt aatcaaaacc cacaacacga
      361 tttcagcgat cttggattgt atccgcgtta ccggtgctac aaaagtcgtc tttaaccacc
      421 tctatgatcc tgtttcgtta gttcgggacc ataccgtaaa ggagaagctg gtggaacgtg
      481 ggatctctgt gcaaagctac aatggagatc tattgtatga accgtgggag atatactgcg
      541 aaaagggcaa accttttacg agtttcaatt cttactggaa gaaatgctta gatatgtcga
      601 ttgaatccgt tatgcttcct cctccttggc ggttgatgcc aataactgca gcggctgaag
      661 cgatttgggc gtgttcgatt gaagaactag ggctggagaa tgaggccgag aaaccgagca
      721 atgcgttgtt aactagagct tggtctccag gatggagcaa tgctgataag ttactaaatg
      781 agttcatcga gaagcagttg atagattatg caaagaacag caagaaagtt gttgggaatt
      841 ctacttcact actttctccg tatctccatt tcggggaaat aagcgtcaga cacgttttcc
      901 agtgtgcccg gatgaa
//
