LOCUS       sequence-111749-         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cytosol-targeted, FRET-based PKA activity reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Herbst KJ, Allen MD, Zhang J
  TITLE     Spatiotemporally regulated protein kinase A activity is a critical 
            regulator of growth factor-stimulated extracellular signal-regulated
            kinase signaling in PC12 cells.
  JOURNAL   Mol Cell Biol. 2011 Oct;31(19):4063-75. doi: 10.1128/MCB.05459-11. 
            Epub 2011 Aug 1.
  PUBMED    21807900
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.05459-11"; journalName: "Mol Cell Biol"; date: 
            "2011-10"; volume: "31"; issue: "19"; pages: "4063-75"
COMMENT     Sequence Label: pcDNA3-AKAR4-NES
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      74..83
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             95..112
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             116..148
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(152..160,161..175)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     complement(227..248)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(488..507)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttgcg gccgccacca tggtgcgggg ttctcatcat catcatcatc atggtatggc
      121 tagcatgact ggtggacagc aaatgggtcg ggatctgtac gacgatgacg ataaggatcc
      181 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
      241 cggcgacgta aacggccaca ggttcagcgt gtccggcgag ggcgagggcg atgccaccta
      301 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
      361 cctcgtgacc accctgacct ggggcgtgca gtgcttcgcc cgctaccccg accacatgaa
      421 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gtaccatctt
      481 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
      541 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
      601 caagctggag tacaacgcca tcagtgacaa cgtctatatc accgccgaca agcagaagaa
      661 cggcatcaag gcccacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
      721 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
      781 ctacctgagc acccagtccg ccctgagcaa
//
