LOCUS       sequence-111750-         377 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses crtEBI genes for neurosporene production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Farasat I, Kushwaha M, Collens J, Easterbrook M, Guido M, Salis HM
  TITLE     Efficient search, mapping, and optimization of multi-protein genetic
            systems in diverse bacteria.
  JOURNAL   Mol Syst Biol. 2014 Jun 21;10:731. doi: 10.15252/msb.20134955.
  PUBMED    24952589
REFERENCE   2  (bases 1 to 377)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2014-06-21"; volume: "10:731. doi"; pages: " 
            10.15252/msb.20134955"
COMMENT     Sequence Label: pBad_crtEBI_14
FEATURES             Location/Qualifiers
     source          1..377
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             61..69
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 cgtctgggta accgtgcgaa cccgatctaa ctcgaagctt aaacccaaca aattagacta
       61 taaggaggtt aataatgcca tctatttctc cagcctctga cgctgatcgt gctctggtta
      121 tcggctctgg cctgggcggc ctggccgctg ctatgcgtct gggtgcaaaa ggttggcgtg
      181 ttaccgtaat cgataaactg gacgttccgg gtggccgtgg ctccagcatc acgcaggagg
      241 gccatcgttt tgacctgggc ccgaccattg ttactgtacc tcaaagcctg cgtgacctgt
      301 ggaaaacttg cggtcgcgac ttcgacgcag atgtggagct gaaaccgatt gacccattct
      361 acgaagttcg ttggcca
//
