LOCUS       sequence-111752-        1109 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Co-expression of human codon-optimized Cas9 nuclease and GFP, 
            plasmid optimized for expression in human pluripotent stem cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Ding Q, Regan SN, Xia Y, Oostrom LA, Cowan CA, Musunuru K
  TITLE     Enhanced efficiency of human pluripotent stem cell genome editing 
            through replacing TALENs with CRISPRs.
  JOURNAL   Cell Stem Cell. 2013 Apr 4;12(4):393-4. doi: 
            10.1016/j.stem.2013.03.006.
  PUBMED    23561441
REFERENCE   2  (bases 1 to 1109)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2013.03"; journalName: "Cell Stem Cell"; date: 
            "2013-04-4- 4"; volume: "12"; issue: "4"; pages: "393-4"
COMMENT     Sequence Label: pCas9_GFP
FEATURES             Location/Qualifiers
     source          1..1109
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gcaaagaatt cgcggccgcc accatggaca agaagtactc cattgggctc gatatcggca
       61 caaacagcgt cggctgggcc gtcattacgg acgagtacaa ggtgccgagc aaaaaattca
      121 aagttctggg caataccgat cgccacagca taaagaagaa cctcattggc gccctcctgt
      181 tcgactccgg ggagacggcc gaagccacgc ggctcaaaag aacagcacgg cgcagatata
      241 cccgcagaaa gaatcggatc tgctacctgc aggagatctt tagtaatgag atggctaagg
      301 tggatgactc tttcttccat aggctggagg agtccttttt ggtggaggag gataaaaagc
      361 acgagcgcca cccaatcttt ggcaatatcg tggacgaggt ggcgtaccat gaaaagtacc
      421 caaccatata tcatctgagg aagaagcttg tagacagtac tgataaggct gacttgcggt
      481 tgatctatct cgcgctggcg catatgatca aatttcgggg acacttcctc atcgaggggg
      541 acctgaaccc agacaacagc gatgtcgaca aactctttat ccaactggtt cagacttaca
      601 atcagctttt cgaagagaac ccgatcaacg catccggagt tgacgccaaa gcaatcctga
      661 gcgctaggct gtccaaatcc cggcggctcg aaaacctcat cgcacagctc cctggggaga
      721 agaagaacgg cctgtttggt aatcttatcg ccctgtcact cgggctgacc cccaacttta
      781 aatctaactt cgacctggcc gaagatgcca agcttcaact gagcaaagac acctacgatg
      841 atgatctcga caatctgctg gcccagatcg gcgaccagta cgcagacctt tttttggcgg
      901 caaagaacct gtcagacgcc attctgctga gtgatattct gcgagtgaac acggagatca
      961 ccaaagctcc gctgagcgct agtatgatca agcgctatga tgagcaccac caagacttga
     1021 ctttgctgaa ggcccttgtc agacagcaac tgcctgagaa gtacaaggaa attttcttcg
     1081 atcagtctaa aaatggctac gccggatac
//
