LOCUS       sequence-111753-         956 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mCherry fused between Cry2 and GRK2ct  to study cell migration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 956)
  AUTHORS   O'Neill PR, Gautam N
  TITLE     Subcellular optogenetic inhibition of G proteins generates signaling
            gradients and cell migration.
  JOURNAL   Mol Biol Cell. 2014 Aug 1;25(15):2305-14. doi: 
            10.1091/mbc.E14-04-0870. Epub 2014 Jun 11.
  PUBMED    24920824
REFERENCE   2  (bases 1 to 956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E14-04-0870"; journalName: "Mol Biol Cell"; date: 
            "2014-08-1- 1"; volume: "25"; issue: "15"; pages: "2305-14"
COMMENT     Sequence Label: Cry2 mCherry GRK2 ct in pcDNA3.1
FEATURES             Location/Qualifiers
     source          1..956
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 agcgagctct ctggctacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
       61 tcactatagg gagacccaag ctggctagcg ctaccggact cagatctcga ggccaccatg
      121 aagatggaca aaaagactat agtttggttt agaagagacc taaggattga ggataatcct
      181 gcattagcag cagctgctca cgaaggatct gtttttcctg tcttcatttg gtgtcctgaa
      241 gaagaaggac agttttatcc tggaagagct tcaagatggt ggatgaaaca atcacttgct
      301 cacttatctc aatccttgaa ggctcttgga tctgacctca ctttaatcaa aacccacaac
      361 acgatttcag cgatcttgga ttgtatccgc gttaccggtg ctacaaaagt cgtctttaac
      421 cacctctatg atcctgtttc gttagttcgg gaccataccg taaaggagaa gctggtggaa
      481 cgtgggatct ctgtgcaaag ctacaatgga gatctattgt atgaaccgtg ggagatatac
      541 tgcgaaaagg gcaaaccttt tacgagtttc aattcttact ggaagaaatg cttagatatg
      601 tcgattgaat ccgttatgct tcctcctcct tggcggttga tgccaataac tgcagcggct
      661 gaagcgattt gggcgtgttc gattgaagaa ctagggctgg agaatgaggc cgagaaaccg
      721 agcaatgcgt tgttaactag agcttggtct ccaggatgga gcaatgctga taagttacta
      781 aatgagttca tcgagaagca gttgatagat tatgcaaaga acagcaagaa agttgttggg
      841 aattctactt cactactttc tccgtatctc catttcgggg aaataagcgt cagacacgtt
      901 ttccagtgtg cccggatgaa acaaattata tgggcaagag ataagaacag tgaagg
//
