LOCUS       sequence-111754-         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FRET-based biosensor for monitoring calcineurin (CaN) phosphatase 
            activity..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Mehta S, Aye-Han NN, Ganesan A, Oldach L, Gorshkov K, Zhang J
  TITLE     Calmodulin-controlled spatial decoding of oscillatory Ca2+ signals 
            by calcineurin.
  JOURNAL   eLife. 2014 Jul 23;3:e03765. doi: 10.7554/eLife.03765.
  PUBMED    25056880
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2014-07-23"; volume: "3:e03765. doi"; pages: " 
            10.7554/eLife.03765"
COMMENT     Sequence Label: pcDNA3-CaNAR2
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      74..83
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             95..112
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             116..148
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(152..160,161..175)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     complement(227..248)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(488..507)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     835..856
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttgcg gccgccacca tggtgcgggg ttctcatcat catcatcatc atggtatggc
      121 tagcatgact ggtggacagc aaatgggtcg ggatctgtac gacgatgacg ataaggatcc
      181 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
      241 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
      301 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
      361 cctcgtgacc accctgagct ggggcgtgca gtgcttcgcc cgctaccccg accacatgaa
      421 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
      481 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
      541 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
      601 caagctggag tacaacgcca tccacggcaa cgtctatatc accgccgaca agcagaagaa
      661 cggcatcaag gccaacttcg gcctcaactg caacatcgag gacggcagcg tgcagctcgc
      721 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
      781 ctacctgagc acccagtcca agctgagcaa agaccccaac gagaagcgcg atcacatggt
      841 cctgctggag ttcgtgaccg ccg
//
