LOCUS       sequence-111756-         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tet inducible knockdown of SOX2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Rudin CM, Durinck S, Stawiski EW, Poirier JT, Modrusan Z, Shames DS,
            Bergbower EA, Guan Y, Shin J, Guillory J, Rivers CS, Foo CK, Bhatt 
            D, Stinson J, Gnad F, Haverty PM, Gentleman R, Chaudhuri S, 
            Janakiraman V, Jaiswal BS, Parikh C, Yuan W, Zhang Z, Koeppen H, Wu 
            TD, Stern HM, Yauch RL, Huffman KE, Paskulin DD, Illei PB, 
            Varella-Garcia M, Gazdar AF, de Sauvage FJ, Bourgon R, Minna JD, 
            Brock MV, Seshagiri S
  TITLE     Comprehensive genomic analysis identifies SOX2 as a frequently 
            amplified gene in small-cell lung cancer.
  JOURNAL   Nat Genet. 2012 Oct;44(10):1111-6. doi: 10.1038/ng.2405. Epub 2012 
            Sep 2.
  PUBMED    22941189
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ng.2405"; 
            journalName: "Nat Genet"; date: "2012-10"; volume: "44"; issue: 
            "10"; pages: "1111-6"
COMMENT     Sequence Label: Tet-pLKO-puro-SOX2
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     301..320
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     primer_bind     complement(403..422)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(404..422)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(450..469)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
ORIGIN
        1 taatgcattc tctagtgaaa aaccttgttg gcataaaaag gctaattgat tttcgagagt
       61 ttcatactgt ttttctgtag gccgtgtacc taaatgtact tttgctccat cgcgatgact
      121 tagtaaagca catctaaaac ttttagcgtt attacgtaaa aaatcttgcc agctttcccc
      181 ttctaaaggg caaaagtgag tatggtgcct atctaacatc tcaatggcta aggcgtcgag
      241 caaagcccgc ttatttttta catgccaata caatgtaggc tgctctacac ctagcttctg
      301 ggcgagttta cgggttgtta aaccttcgat tccgacctca ttaagcagct ctaatgcgct
      361 gttaatcact ttacttttat ctaatctaga catatcaatt cgccctatag tgagtcgtat
      421 tacaattctt tgccaaaatg atgagacagc acaataacca gcacgttgcc caggagctgt
      481 aggaaaaaga agaaggcatg aacatggtta gcagaggggc ccggtttgga ctcagagtat
      541 tttatcctca tctcaaacag tgtatatcat tgtaaccata aagagaaagg caggatgatg
      601 accaggatgt agttgtttct accaataaga atatttccac gccagccaga atttatatgc
      661 agaaatattc taccttatca tttaattata acaattgttc tctaaaactg tgctgaagta
      721 caatataata taccctgatt gccttgaaaa aaaagtgatt agagaaagta cttacaatct
      781 gacaaataa
//
