LOCUS       sequence-111758-         976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mCherry fused between Cry2 and GRK2ct  to study cell migration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 976)
  AUTHORS   O'Neill PR, Gautam N
  TITLE     Subcellular optogenetic inhibition of G proteins generates signaling
            gradients and cell migration.
  JOURNAL   Mol Biol Cell. 2014 Aug 1;25(15):2305-14. doi: 
            10.1091/mbc.E14-04-0870. Epub 2014 Jun 11.
  PUBMED    24920824
REFERENCE   2  (bases 1 to 976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E14-04-0870"; journalName: "Mol Biol Cell"; date: 
            "2014-08-1- 1"; volume: "25"; issue: "15"; pages: "2305-14"
COMMENT     Sequence Label: Cry2 mCherry GRK2 ct in pcDNA3.1
FEATURES             Location/Qualifiers
     source          1..976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(921..940)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gagcggccgc cactgtgctg gatatctgca gaattctcag aggccgttgg cactgccgcg
       61 ctggatcagt ggcaccttgc tcagctccac gacgggcgag cgcggcttgt tcttcatctt
      121 gggcacccgc tgcactagct gctgggcctc gcggtaggcg tctcgaagct ccttcttcca
      181 ctgcaccagc tctgggtcac tatcgcactg caggacaaac tgcttgccac ctcggatctt
      241 gaggaggagg cactttcgct ccttgatctg cgtctcctcc accgactgga tctcctccat
      301 ggtcagcagg ctctgcgggg cctcgccctc gccccgccac tcgagccggt tagggaacag
      361 gtagaagtac cgccgctgcc actgggtcag gaaggggttg cccatcttgg acatgtagcc
      421 atgcatgatg cagtccttgc ccagggcgta gtcttcctcg tggcccaact gcttgttttt
      481 ggttttcttg cgggcctcca gccggtccgt ctcagcattg atggtgtcaa agacagtctc
      541 tgctacctcc tgctgccacc gctccgagat ggtcaggggg aagttgcggt agagctcctg
      601 gtcactgtcc agtagcttga ttcccatgga tccgagctcg gtacccttgt acagctcgtc
      661 catgccgccg gtggagtggc ggccctcggc gcgttcgtac tgttccacga tggtgtagtc
      721 ctcgttgtgg gaggtgatgt ccaacttgat gttgacgttg taggcgccgg gcagctgcac
      781 gggcttcttg gccttgtagg tggtcttgac ctcagcgtcg tagtggccgc cgtccttcag
      841 cttcagcctc tgcttgatct cgcccttcag gcgccgtcct cggggtacat ccgctcggag
      901 gaggcctccc agcccatggg tcttcttctg cattacgggg ccgtcggagg gaaattggtg
      961 ccgcgcaact tcacct
//
