LOCUS       sequence-111759-        1120 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 expression cassette carried by a yeast single-copy episome 
            plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Zhang GC, Kong II, Kim H, Liu JJ, Cate JH, Jin YS
  TITLE     Construction of a quadruple auxotrophic mutant of an industrial 
            polyploid saccharomyces cerevisiae strain by using RNA-guided Cas9 
            nuclease.
  JOURNAL   Appl Environ Microbiol. 2014 Dec;80(24):7694-701. doi: 
            10.1128/AEM.02310-14. Epub 2014 Oct 3.
  PUBMED    25281382
REFERENCE   2  (bases 1 to 1120)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/AEM.02310-14"; journalName: "Appl Environ Microbiol"; date:
            "2014-12"; volume: "80"; issue: "24"; pages: "7694-701"
COMMENT     Sequence Label: Cas9-NAT
FEATURES             Location/Qualifiers
     source          1..1120
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      538..735
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 cagtgtcccg ggggacgccg aggccatcga ggcactggat gggtccttca ccaccgacac
       61 cgtcttccgc gtcaccgcca ccggggacgg cttcaccctg cgggaggtgc cggtggaccc
      121 gcccctgacc aaggtgttcc ccgacgacga atcggacgac gaatcggacg acggggagga
      181 cggcgacccg gactcccgga cgttcgtcgc gtacggggac gacggcgacc tggcgggctt
      241 cgtggtcgtc tcgtactccg gctggaaccg ccggctgacc gtcgaggaca tcgaggtcgc
      301 cccggagcac cgggggcacg gggtcgggcg cgcgttgatg gggctcgcga cggagttcgc
      361 ccgcgagcgg ggcgccgggc acctctggct ggaggtcacc aacgtcaacg caccggcgat
      421 ccacgcgtac cggcggatgg ggttcaccct ctgcggcctg gacaccgccc tgtacgacgg
      481 caccgcctcg gacggcgagc aggcgctcta catgagcatg ccctgcccct aatcagtact
      541 gacaataaaa agattcttgt tttcaagaac ttgtcatttg tatagttttt ttatattgta
      601 gttgttctat tttaatcaaa tgttagcgtg atttatattt tttttcgcct cgacatcatc
      661 tgcccagatg cgaagttaag tgcgcagaaa gtaatatcat gcgtcaatcg tatgtgaatg
      721 ctggtcgcta tactgctgtc gattcgatac taacgccgcc atccagtgtc gaaaacgcaa
      781 ttgacccggt tattgcaagg aaaatttcaa gtcttgtaaa agcatataaa aatagttcag
      841 gcactccgaa atacttggtt ggcgtgtttc gtaatcaacc taaggaggat gttttggctc
      901 tggtcaatga ttacggcatt gatatcgtcc aactgcatgg agatgagtcg tggcaagaat
      961 accaagagtt cctcggtttg ccagttatta aaagactcgt atttccaaaa gactgcaaca
     1021 tactactcag tgcagcttca cagaaacctc attcgtttat tcccttgttt gattcagaag
     1081 caggtgggac aggtgaactt ttggattgga actcgatttc
//
