LOCUS       sequence-111764-        7276 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  wt VP2/VP3 only to participate in capsid assembly; no ITR's.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7276)
  AUTHORS   Judd J, Wei F, Nguyen PQ, Tartaglia LJ, Agbandje-McKenna M, Silberg 
            JJ, Suh J
  TITLE     Random Insertion of mCherry Into VP3 Domain of Adeno-associated 
            Virus Yields Fluorescent Capsids With no Loss of Infectivity.
  JOURNAL   Mol Ther Nucleic Acids. 2012 Nov 13;1:e54. doi: 
            10.1038/mtna.2012.46.
  PUBMED    23629029
REFERENCE   2  (bases 1 to 7276)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Nucleic Acids."; date: "2012-11-13"; volume: "1:e54. doi"; pages: " 
            10.1038/mtna.2012.46"
COMMENT     Sequence Label: pRC_RR_VP2/3
FEATURES             Location/Qualifiers
     source          1..7276
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     669..685
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     670..686
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(720..736)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(720..736)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             4798..4809
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(5088..5108)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5088..5106)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5127..5143)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5127..5143)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5140..5162)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5151..5167
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5175..5181,5182..5199,5200..5205))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5220..5241
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5358..5375)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5529..6117)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5609..5628)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6288..7079,7080..7148))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6911..6930
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7149..7253)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg
      661 gccccccctc gaggtcgacg gtatcgataa gcttgatatc gaattcctgc agcccggggg
      721 atccactagt tctagagtcc tgtattagag gtcacgtgag tgttttgcga cattttgcga
      781 caccatgtgg tcacgctggg tatttaagcc cgagtgagca cgcagggtct ccattttgaa
      841 gcgggaggtt tgaacgcgca gccgccatgc cggggtttta cgagattgtg attaaggtcc
      901 ccagcgacct tgacgagcat ctgcccggca tttctgacag ctttgtgaac tgggtggccg
      961 agaaggaatg ggagttgccg ccagattctg acatggatct gaatctgatt gagcaggcac
     1021 ccctgaccgt ggccgagaag ctgcagcgcg actttctgac ggaatggcgt cgtgtgagta
     1081 aggccccgga ggcccttttc tttgtgcaat ttgagaaggg agagagctac ttccacatgc
     1141 acgtgctcgt ggaaaccacc ggggtgaaat ctatggtttt gggacgtttc ctgagtcaga
     1201 ttcgcgaaaa actgattcag agaatttacc gcgggatcga gccgactttg ccaaactggt
     1261 tcgcggtcac aaagaccaga aatggtgccg gaggcgggaa caaggtggtg gatgagtgct
     1321 acatccccaa ttacttgctc cccaaaaccc agcctgagct ccagtgggcg tggactaata
     1381 tggaacagta tttaagcgcc tgtttgaatc tcacggagcg taaacggttg gtggcgcagc
     1441 atctgacgca cgtgtcgcag acgcaggagc agaacaaaga gaatcagaat cccaattctg
     1501 atgcgccggt gatcagatca aaaacttcag ccaggtacat ggagctggtc gggtggctcg
     1561 tggacaaggg gattacctcg gagaagcagt ggatacagga ggaccaggcc tcatacatct
     1621 ccttcaatgc ggcctccaac tcgcggtccc aaatcaaggc tgccttggac aatgcgggaa
     1681 agattatgag cctgactaaa accgcccccg actacctggt gggccagcag cccgtggagg
     1741 acatttccag caatcggatt tataaaattt tggaactaaa cgggtacgat ccccaatatg
     1801 cggcttccgt ctttctggga tgggccacga aaaagttcgg caagaggaac accatctggc
     1861 tgtttgggcc tgcaactacc gggaagacca acatcgcgga ggccatagcc cacactgtgc
     1921 ccttctacgg gtgcgtaaac tggaccaatg agaactttcc cttcaacgac tgtgtcgaca
     1981 agatggtgat ctggtgggag gaggggaaga tgaccgccaa ggtcgtggag tcggccaaag
     2041 ccattctcgg aggaagcaag gtgcgcgtgg accagaaatg caagtcctcg gcccagatag
     2101 acccgactcc cgtgatcgtc acctccaaca ccaacatgtg cgccgtgatt gacgggaact
     2161 caacgacctt cgaacaccag cagccgttgc aagaccggat gttcaaattt gaactcaccc
     2221 gccgtctgga tcatgacttt gggaaggtca ccaagcagga agtcaaagac tttttccggt
     2281 gggcaaagga tcacgtggtt gaggtggagc atgaattcta cgtcaaaaag ggtggagcca
     2341 agaaaagacc cgcccccagt gacgcagata taagtgagcc caaacgggtg cgcgagtcag
     2401 ttgcgcagcc atcgacgtca gacgcggaag cttcgatcaa ctacgcagac aggtaccaaa
     2461 acaaatgttc tcgtcacgtg ggcatgaatc tgatgctgtt tccctgcaga caatgcgaga
     2521 gaatgaatca gaattcaaat atctgcttca ctcacggaca gaaagactgt ttagagtgct
     2581 ttcccgtgtc agaatctcaa cccgtttctg tcgtcaaaaa ggcgtatcag aaactgtgct
     2641 acattcatca tatcatggga aaggtgccag acgcttgcac tgcctgcgat ctggtcaatg
     2701 tggatttgga tgactgcatc tttgaacaat aaatgattta aatcaggtaa ggctgccgat
     2761 ggttatcttc cagattggct cgaggacact ctctctgaag gaataagaca gtggtggaag
     2821 ctcaaacctg gcccaccacc accaaagccc gcagagcggc ataaggacga cagcaggggt
     2881 cttgtgcttc ctgggtacaa gtacctcgga cccttcaacg gactcgacaa gggagagccg
     2941 gtcaacgagg cagacgccgc ggccctcgag cacgacaaag cctacgaccg gcagctcgac
     3001 agcggagaca acccgtacct caagtacaac cacgccgacg cggagtttca ggagcgcctt
     3061 aaagaagata cgtcttttgg gggcaacctc ggacgagcag tcttccaggc gaaaaagagg
     3121 gttcttgaac ctctgggcct ggttgaggaa cctgttaaga cggctccggg aaaaaagagg
     3181 ccggtagagc actctcctgt ggagccagac tcctcctcgg gaaccggaaa ggcgggccag
     3241 cagcctgcaa gaaaaagatt gaattttggt cagactggag acgcagactc agtacctgac
     3301 ccccagcctc tcggacagcc accagcagcc ccctctggtc tgggaactaa taccatggct
     3361 acaggcagtg gcgcaccaat ggcagacaat aacgagggtg ccgacggagt gggtaattcc
     3421 tcgggaaatt ggcattgcga ttccacatgg atgggcgaca gagtcatcac caccagcacc
     3481 cgaacctggg ccctgcccac ctacaacaac cacctctaca aacaaatttc cagccaatca
     3541 ggagcctcga acgacaatca ctactttggc tacagcaccc cttgggggta ttttgacttc
     3601 aacagattcc actgccactt ttcaccacgt gactggcaaa gactcatcaa caacaactgg
     3661 ggattccgac ccaagagact caacttcaag ctctttaaca ttcaagtcaa agaggtcacg
     3721 cagaatgacg gtacgacgac gattgccaat aaccttacca gcacggttca ggtgtttact
     3781 gactcggagt accagctccc gtacgtcctc ggctcggcgc atcaaggatg cctcccgccg
     3841 ttcccagcag acgtcttcat ggtgccacag tatggatacc tcaccctgaa caacgggagt
     3901 caggcagtag gacgctcttc attttactgc ctggagtact ttccttctca gatgctgcgt
     3961 accggaaaca actttacctt cagctacact tttgaggacg ttcctttcca cagcagctac
     4021 gctcacagcc agagtctgga ccgtctcatg aatcctctca tcgaccagta cctgtattac
     4081 ttgagcagaa caaacactcc aagtggaacc accacgcagt caaggcttca gttttctcag
     4141 gccggagcga gtgacattcg ggaccagtct aggaactggc ttcctggacc ctgttaccgc
     4201 cagcagcgag tatcaaagac atctgcggat aacaacaaca gtgaatactc gtggactgga
     4261 gctaccaagt accacctcaa tggcagagac tctctggtga atccgggccc ggctatggca
     4321 agccacaagg acgatgaaga aaagtttttt cctcagagcg gggttctcat ctttgggaag
     4381 caaggctcag agaaaacaaa tgtggacatt gaaaaggtca tgattacaga cgaagaggaa
     4441 atcaggacaa ccaatcccgt ggctacggag cagtatggtt ctgtatctac caacctccag
     4501 agaggcaaca gacaagcagc taccgcagat gtcaacacac aaggcgttct tccaggcatg
     4561 gtctggcagg acagagatgt gtaccttcag gggcccatct gggcaaagat tccacacacg
     4621 gacggacatt ttcacccctc tcccctcatg ggtggattcg gacttaaaca ccctcctcca
     4681 cagattctca tcaagaacac cccggtgcct gcgaatcctt cgaccacctt cagtgcggca
     4741 aagtttgctt ccttcatcac acagtactcc acgggacagg tcagcgtgga gatcgagtgg
     4801 gagctgcaga aggaaaacag caaacgctgg aatcccgaaa ttcagtacac ttccaactac
     4861 aacaagtctg ttaatgtgga ctttactgtg gacactaatg gcgtgtattc agagcctcgc
     4921 cccattggca ccagatacct gactcgtaat ctgtaattgg caggtcttgt taatcaataa
     4981 accgtttaat tcgtttcagt tgaactttgg tctctgcgta tttctttctt atctagtttc
     5041 catgctctag agcggccgcc accgcggtgg agctccagct tttgttccct ttagtgaggg
     5101 ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     5161 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     5221 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     5281 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     5341 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     5401 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     5461 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     5521 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     5581 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     5641 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     5701 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     5761 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     5821 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     5881 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     5941 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     6001 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     6061 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     6121 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     6181 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     6241 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     6301 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     6361 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     6421 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     6481 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     6541 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     6601 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     6661 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     6721 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     6781 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     6841 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     6901 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     6961 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     7021 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     7081 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     7141 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     7201 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     7261 ccccgaaaag tgccac
//
