LOCUS       sequence-111767-         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mitochondria-targeted FRET biosensor for monitoring calcineurin 
            (CaN) phosphatase activity; targeted to cytosolic face of 
            mitochondrial outer membrane..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Mehta S, Aye-Han NN, Ganesan A, Oldach L, Gorshkov K, Zhang J
  TITLE     Calmodulin-controlled spatial decoding of oscillatory Ca2+ signals 
            by calcineurin.
  JOURNAL   eLife. 2014 Jul 23;3:e03765. doi: 10.7554/eLife.03765.
  PUBMED    25056880
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2014-07-23"; volume: "3:e03765. doi"; pages: " 
            10.7554/eLife.03765"
COMMENT     Sequence Label: pcDNA3-Mito-CaNAR2
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      68..77
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(218..239)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(479..498)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttgcc accatggcaa tccagttgcg ttcgctcttc cccttggcgt tgcccggaat
      121 gctggccctc cttggctggt ggtggttttt ctctcgtaaa aaagcggatc ccatggtgag
      181 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
      241 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
      301 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
      361 caccctgagc tggggcgtgc agtgcttcgc ccgctacccc gaccacatga agcagcacga
      421 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
      481 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
      541 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
      601 gtacaacgcc atccacggca acgtctatat caccgccgac aagcagaaga acggcatcaa
      661 ggccaacttc ggcctcaact gcaacatcga ggacggcagc gtgcagctcg ccgaccacta
      721 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
      781 cacccagtcc aagctga
//
