LOCUS       sequence-111788-         998 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bicistronic expression of Centrin-mCherry fusion and Puro resistance
            gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 998)
  TITLE     Piel Lab Plasmids
REFERENCE   2  (bases 1 to 998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pIRES Centrin1 Cherry Puro
FEATURES             Location/Qualifiers
     source          1..998
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    259..380
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(296..315)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(481..497)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(481..497)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(494..516)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    505..521
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(529..535,536..553,554..559))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    574..595
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(712..729)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(963..982)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgacccgc aagcccggtg cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag
       61 gagcgcacga ccccatggct ccgaccgaag ccacccgggg cggccccgcc gaccccgcac
      121 ccgcccccga ggcccaccga ctctagataa ctgatcataa tcagccatac cacatttgta
      181 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
      241 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
      301 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
      361 aaactcatca atgtatctta acgctcgagt gcattctagt tgtggtttgt ccaaactcat
      421 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
      481 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
      541 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
      601 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
      661 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
      721 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
      781 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
      841 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
      901 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
      961 ataaagatac caggcgtttc cccctggaag ctccctcg
//
