LOCUS       sequence-111790-        1044 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bicistronic expression of Centrin-GFP fusion and Puro resistance 
            gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1044)
  TITLE     Piel Lab Plasmids
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pIRES Centrin1 GFP Pur
FEATURES             Location/Qualifiers
     source          1..1044
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    270..391
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(307..326)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(492..508)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(492..508)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(505..527)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    516..532
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(540..546,547..564,565..570))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    585..606
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(723..740)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(976..995)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcgcactggt gcatgacccg caagcccggt gcctgacgcc cgccccacga cccgcagcgc
       61 ccgaccgaaa ggagcgcacg accccatggc tccgaccgaa gccacccggg gcggccccgc
      121 cgaccccgca cccgcccccg aggcccaccg actctagata actgatcata atcagccata
      181 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
      241 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
      301 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
      361 gtggtttgtc caaactcatc aatgtatctt aacgctcgag tgcattctag ttgtggtttg
      421 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
      481 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
      541 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
      601 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
      661 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
      721 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
      781 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
      841 gcaaaaggcc agcaaaaagg ccaggaaacc gtaaaaaggc cgcgttgctg gcgtttttcc
      901 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
      961 acccgacagg actataaaga taccaggcgt ttccccctgg aagctcccct cgggggctct
     1021 cctgttccga accctggcgc ttac
//
