LOCUS       sequence-111794-        1005 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Burkel BM, von Dassow G, Bement WM
  TITLE     Versatile fluorescent probes for actin filaments based on the 
            actin-binding domain of utrophin.
  JOURNAL   Cell Motil Cytoskeleton. 2007 Nov . 64(11):822-32.
  PUBMED    17685442
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Motil 
            Cytoskeleton. 2007 Nov . 64(11):822-32."
COMMENT     Sequence Label: mCherry-UtrCH
FEATURES             Location/Qualifiers
     source          1..1005
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..711
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(149..167)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     417..436
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 accatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc
       61 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
      121 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg
      181 cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag
      241 caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag
      301 cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag
      361 gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc
      421 gtaatgcaga agaagaccat gggctgggag gcctcctccg agagaatgta ccccgaggac
      481 ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac
      541 gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac
      601 gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
      661 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gtccggaacc
      721 atggccaagt atggagaaca tgaagccagt cctgacaatg ggcagaacga attcagtgat
      781 atcattaagt ccagatctga tgaacacaat gacgtacaga agaaaacctt taccaaatgg
      841 ataaatgctc gattttcaaa gagtgggaaa ccacccatca atgatatgtt cacagacctc
      901 aaagatggaa ggaagctatt ggatcttcta gaaggcctca caggaacatc actgccaaag
      961 gaacgtggtt ccacaagggt acatgcctta aataacgtca acaga
//
