LOCUS       sequence-111796-        1520 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Burkel BM, von Dassow G, Bement WM
  TITLE     Versatile fluorescent probes for actin filaments based on the 
            actin-binding domain of utrophin.
  JOURNAL   Cell Motil Cytoskeleton. 2007 Nov . 64(11):822-32.
  PUBMED    17685442
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Motil 
            Cytoskeleton. 2007 Nov . 64(11):822-32."
COMMENT     Sequence Label: mCherry-UtrCH
FEATURES             Location/Qualifiers
     source          1..1520
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             12..719
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(157..175)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     425..444
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ggatccaaac catggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca
       61 tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg
      121 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg
      181 gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct
      241 acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca
      301 agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct
      361 ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg
      421 acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag agaatgtacc
      481 ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc
      541 actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg
      601 cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg
      661 aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt
      721 ccggaaccat ggccaagtat ggagaacatg aagccagtcc tgacaatggg cagaacgaat
      781 tcagtgatat cattaagtcc agatctgatg aacacaatga cgtacagaag aaaaccttta
      841 ccaaatggat aaatgctcga ttttcaaaga gtgggaaacc acccatcaat gatatgttca
      901 cagacctcaa agatggaagg aagctattgg atcttctaga aggcctcaca ggaacatcac
      961 tgccaaagga acgtggttcc acaagggtac atgccttaaa taacgtcaac agagtgctgc
     1021 aggttttaca tcagaacaat gtggaattag tgaatatagg gggaaccgac attgtggatg
     1081 gaaatcacaa actgactttg gggttacttt ggagcatcat tttgcactgg caggtgaaag
     1141 atgtcatgaa ggatgtcatg tcggacctgc agcagacgaa cagtgagaag atcctgctca
     1201 gctgggtgcg tcagaccacc aggccctaca gccaagtcaa cgtcctcaac ttcaccacca
     1261 gctggacaga tggactcgcc tttaatgctg tcctccaccg acataaacct gatctcttca
     1321 gctgggataa agttgtcaaa atgtcaccaa ttgagagact tgaacatgcc ttcagcaagg
     1381 ctcaaactta tttgggaatt gaaaagctgt tagatcctga agatgttgcc gttcggcttc
     1441 ctgacaagaa atccataatt atgtatttaa catctttgtt tgaggtgcta cctcagcaag
     1501 tcaccataga ctaactcgag
//
