LOCUS       sequence-111797-         960 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bicistronic expression of GFP-Utrophin and Neo resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 960)
  TITLE     Piel Lab Plasmids
REFERENCE   2  (bases 1 to 960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pIRESneo3+GFP-Utr_CH
FEATURES             Location/Qualifiers
     source          1..960
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    211..332
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(248..267)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(434..450)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(434..450)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(447..469)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    458..474
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(482..488,489..506,507..512))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    527..548
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(665..682)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(916..935)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
       61 cttcttgacg agttcttctg aggggatcaa ttctctagat aactgatcat aatcagccat
      121 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
      181 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
      241 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
      301 tgtggtttgt ccaaactcat caatgtatct taacgcgtcg agtgcattct agttgtggtt
      361 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct
      421 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
      481 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
      541 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
      601 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
      661 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
      721 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
      781 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
      841 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
      901 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
//
