LOCUS       sequence-11180-e        2058 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
            Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, 
            Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao 
            JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
            Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, 
            Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
  TITLE     COT drives resistance to RAF inhibition through MAP kinase pathway 
            reactivation.
  JOURNAL   Nature. 2010 Dec 16;468(7326):968-72. doi: 10.1038/nature09627
  PUBMED    21107320
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-12-16- 16"; volume: "468"; issue: "7326"; 
            pages: "968-72"
COMMENT     Sequence Label: pDONR223-PLK2
FEATURES             Location/Qualifiers
     source          1..2058
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             169..186
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atggagcttt tgcggactat cacctaccag ccagccgcca gcaccaaaat gtgcgagcag
       61 gcgctgggca agggttgcgg agcagactcg aagaagaagc ggccgccgca gccccccgag
      121 gaatcgcagc cacctcagtc ccaggcgcaa gtgcccccgg cggcccctca ccaccatcac
      181 caccattcgc actcggggcc ggagatctcg cggattatcg tcgaccccac gactgggaag
      241 cgctactgcc ggggcaaagt gctgggaaag ggtggctttg caaaatgtta cgagatgaca
      301 gatttgacaa ataacaaagt ctacgccgca aaaattattc ctcacagcag agtagctaaa
      361 cctcatcaaa gggaaaagat tgacaaagaa atagagcttc acagaattct tcatcataag
      421 catgtagtgc agttttacca ctacttcgag gacaaagaaa acatttacat tctcttggaa
      481 tactgcagta gaaggtcaat ggctcatatt ttgaaagcaa gaaaggtgtt gacagagcca
      541 gaagttcgat actacctcag gcagattgtg tctggactga aataccttca tgaacaagaa
      601 atcttgcaca gagatctcaa actagggaac ttttttatta atgaagccat ggaactaaaa
      661 gttggggact tcggtctggc agccaggcta gaacccttgg aacacagaag gagaacgata
      721 tgtggtaccc caaattatct ctctcctgaa gtcctcaaca aacaaggaca tggctgtgaa
      781 tcagacattt gggccctggg ctgtgtaatg tatacaatgt tactagggag gcccccattt
      841 gaaactacaa atctcaaaga aacttatagg tgcataaggg aagcaaggta tacaatgccg
      901 tcctcattgc tggctcctgc caagcactta attgctagta tgttgtccaa aaacccagag
      961 gatcgtccca gtttggatga catcattcga catgactttt ttttgcaggg cttcactccg
     1021 gacagactgt cttctagctg ttgtcataca gttccagatt tccacttatc aagcccagct
     1081 aagaatttct ttaagaaagc agctgctgct ctttttggtg gcaaaaaaga caaagcaaga
     1141 tatattgaca cacataatag agtgtctaaa gaagatgaag acatctacaa gcttaggcat
     1201 gatttgaaaa agacttcaat aactcagcaa cccagcaaac acaggacaga tgaggagctc
     1261 cagccaccta ccaccacagt tgccaggtct ggaacacccg cagtagaaaa caagcagcag
     1321 attggggatg ctattcggat gatagtcaga gggactcttg gcagctgtag cagcagcagt
     1381 gaatgccttg aagacagtac catgggaagt gttgcagaca cagtggcaag ggttcttcgg
     1441 ggatgtctgg aaaacatgcc ggaagctgat tgcattccca aagagcagct gagcacatca
     1501 tttcagtggg tcaccaaatg ggttgattac tctaacaaat atggctttgg gtaccagctc
     1561 tcagaccaca ccgtcggtgt ccttttcaac aatggtgctc acatgagcct ccttccagac
     1621 aaaaaaacag ttcactatta cgcagagctt ggccaatgct cagttttccc agcaacagat
     1681 gctcctgagc aatttattag tcaagtgacg gtgctgaaat acttttctca ttacatggag
     1741 gagaacctca tggatggtgg agatctgcct agtgttactg atattcgaag acctcggctc
     1801 tacctccttc agtggctaaa atctgataag gccctaatga tgctctttaa tgatggcacc
     1861 tttcaggtga atttctacca tgatcataca aaaatcatca tctgtagcca aaatgaagaa
     1921 taccttctca cctacatcaa tgaggatagg atatctacaa ctttcaggct gacaactctg
     1981 ctgatgtctg gctgttcatc agaattaaaa aatcgaatgg aatatgccct gaacatgctc
     2041 ttacaaagat gtaactga
//
