LOCUS       sequence-111806-         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of (-30)GFP-9xGGS-Cre-6xHis in bacterial cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Zuris JA, Thompson DB, Shu Y, Guilinger JP, Bessen JL, Hu JH, Maeder
            ML, Joung JK, Chen ZY, Liu DR
  TITLE     Cationic lipid-mediated delivery of proteins enables efficient 
            protein-based genome editing in vitro and in vivo.
  JOURNAL   Nat Biotechnol. 2015 Jan;33(1):73-80. doi: 10.1038/nbt.3081. Epub 
            2014 Oct 30.
  PUBMED    25357182
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3081"; 
            journalName: "Nat Biotechnol"; date: "2015-01"; volume: "33"; issue:
            "1"; pages: "73-80"
COMMENT     Sequence Label: pET-(-30)GFP-9xGGS-Cre-6xHis
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     57..76
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        57..75
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    76..100
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             115..137
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 gccggccacg atgcgtccgg cgtagaggat cgagatcgat ctcgatcccg cgaaattaat
       61 acgactcact ataggggaat tgtgagcgga taacaattcc cctctagaaa taattttgtt
      121 taactttaag aaggagatat accatgggtg ctagcaaagg tgaagagctg tttgacggtg
      181 tagtaccgat cttagtggaa ttagacggcg acgtgaacgg tcacgaattt agcgtgcgcg
      241 gcgagggcga aggtgacgct accgagggtg aattgaccct gaagtttatt tgcacaacag
      301 gcgaattacc cgttccgtgg cccaccttag tgaccaccct gacctatggc gttcagtgct
      361 tcagtgatta cccagatcat atggatcaac acgatttttt caaatcagcc atgcctgaag
      421 gatatgttca agagcgtaca atcagcttca aggacgatgg cacctataaa acgcgtgcgg
      481 aagtgaaatt tgaaggcgac acattagtaa accgtatcga actgaaaggt atcgacttca
      541 aagaagacgg caacatttta ggccataagc tggaatataa ctttaattct catgacgtgt
      601 atattacggc cgataaacag gaaaacggta tcaaggcaga atttgaaatt cgccataacg
      661 tggaggacgg cagcgttcaa ttagcggatc attatcaaca aaacacgccg attggtgatg
      721 ggcctgtact gttacctgac gatcactacc tgagcacgga gtcagccctg agcaaagatc
      781 cgaacgaaga ccgcgatcac atggttctgt tagaattcgt gaccgctgca ggcattgatc
      841 atggaatgga cgagctgtac aagaccggtg gtagcggtgg ttctggtggt tctggtggta
      901 gcggcggt
//
