LOCUS       sequence-111820-         992 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mCherry fused between Cry2 and RGS4(del 1-33) to study cell 
            migration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 992)
  AUTHORS   O'Neill PR, Gautam N
  TITLE     Subcellular optogenetic inhibition of G proteins generates signaling
            gradients and cell migration.
  JOURNAL   Mol Biol Cell. 2014 Aug 1;25(15):2305-14. doi: 
            10.1091/mbc.E14-04-0870. Epub 2014 Jun 11.
  PUBMED    24920824
REFERENCE   2  (bases 1 to 992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E14-04-0870"; journalName: "Mol Biol Cell"; date: 
            "2014-08-1- 1"; volume: "25"; issue: "15"; pages: "2305-14"
COMMENT     Sequence Label: Cry2 mCherry RGS4(del 1-33) in pcDNA3.1
FEATURES             Location/Qualifiers
     source          1..992
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gagctctctg gctactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctg gctagcgcta ccggactcag atctcgaggc caccatgaag
      121 atggacaaaa agactatagt ttggtttaga agagacctaa ggattgagga taatcctgca
      181 ttagcagcag ctgctcacga aggatctgtt tttcctgtct tcatttggtg tcctgaagaa
      241 gaaggacagt tttatcctgg aagagcttca agatggtgga tgaaacaatc acttgctcac
      301 ttatctcaat ccttgaaggc tcttggatct gacctcactt taatcaaaac ccacaacacg
      361 atttcagcga tcttggattg tatccgcgtt accggtgcta caaaagtcgt ctttaaccac
      421 ctctatgatc ctgtttcgtt agttcgggac cataccgtaa aggagaagct ggtggaacgt
      481 gggatctctg tgcaaagcta caatggagat ctattgtatg aaccgtggga gatatactgc
      541 gaaaagggca aaccttttac gagtttcaat tcttactgga agaaatgctt agatatgtcg
      601 attgaatccg ttatgcttcc tcctccttgg cggttgatgc caataactgc agcggctgaa
      661 gcgatttggg cgtgttcgat tgaagaacta gggctggaga atgaggccga gaaaccgagc
      721 aatgcgttgt taactagagc ttggtctcca ggatggagca atgctgataa gttactaaat
      781 gagttcatcg agaagcagtt gatagattat gcaaagaaca gcaagaaagt tgttgggaat
      841 tctacttcac tactttctcc gtatctccat ttcggggaaa taagcgtcag acacgttttc
      901 cagtgtgccc ggatgaaaca aattatatgg gcaagagata agaacagtga aggagaagaa
      961 agtgcagatc tttttcttag gggaatcggt tt
//
