LOCUS       sequence-111824-        1002 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mCherry fused between Cry2 and PGK-1 to study cell migration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   O'Neill PR, Gautam N
  TITLE     Subcellular optogenetic inhibition of G proteins generates signaling
            gradients and cell migration.
  JOURNAL   Mol Biol Cell. 2014 Aug 1;25(15):2305-14. doi: 
            10.1091/mbc.E14-04-0870. Epub 2014 Jun 11.
  PUBMED    24920824
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E14-04-0870"; journalName: "Mol Biol Cell"; date: 
            "2014-08-1- 1"; volume: "25"; issue: "15"; pages: "2305-14"
COMMENT     Sequence Label: Cry2 mCherry PGK-1 in pcDNA3.1
FEATURES             Location/Qualifiers
     source          1..1002
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gctctctggc tactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagctggc tagcgctacc ggactcagat ctcgaggcca ccatgaagat
      121 ggacaaaaag actatagttt ggtttagaag agacctaagg attgaggata atcctgcatt
      181 agcagcagct gctcacgaag gatctgtttt tcctgtcttc atttggtgtc ctgaagaaga
      241 aggacagttt tatcctggaa gagcttcaag atggtggatg aaacaatcac ttgctcactt
      301 atctcaatcc ttgaaggctc ttggatctga cctcacttta atcaaaaccc acaacacgat
      361 ttcagcgatc ttggattgta tccgcgttac cggtgctaca aaagtcgtct ttaaccacct
      421 ctatgatcct gtttcgttag ttcgggacca taccgtaaag gagaagctgg tggaacgtgg
      481 gatctctgtg caaagctaca atggagatct attgtatgaa ccgtgggaga tatactgcga
      541 aaagggcaaa ccttttacga gtttcaattc ttactggaag aaatgcttag atatgtcgat
      601 tgaatccgtt atgcttcctc ctccttggcg gttgatgcca ataactgcag cggctgaagc
      661 gatttgggcg tgttcgattg aagaactagg gctggagaat gaggccgaga aaccgagcaa
      721 tgcgttgtta actagagctt ggtctccagg atggagcaat gctgataagt tactaaatga
      781 gttcatcgag aagcagttga tagattatgc aaagaacagc aagaaagttg ttgggaattc
      841 tacttcacta ctttctccgt atctccattt cggggaaata agcgtcagac acgttttcca
      901 gtgtgcccgg atgaaacaaa ttatatgggc aagagataag aacagtgaag gagaagaaag
      961 tgcagatctt tttcttaggg gaatcggttt aagagagtat tc
//
