LOCUS       sequence-111830-         707 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  2nd generation plasmid for phage display on modified geneIII, 
            contains stuffer fragment.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Barbas CF, Wagner J
  TITLE     Synthetic Human Antibodies: Selecting and Evolving Functional 
            Proteins Methods, A companion to Methods in Enzymology 8, 94-103
  JOURNAL   Methods, A companion to Methods in Enzymology, 1995; 8(2): 94-103.
REFERENCE   2  (bases 1 to 707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods, A 
            companion to Methods in Enzymology,"; date: "1995"; volume: " 8(2)";
            pages: " 94-103"
COMMENT     Sequence Label: pComb3HSS
FEATURES             Location/Qualifiers
     source          1..707
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     complement(54..119)
                     /codon_start=1
                     /gene="pelB (fragment)"
                     /product="leader peptide for secretion"
                     /label=pelB signal sequence
                     /translation="MKYLLPTAAAGLLLLAAQPAMA"
ORIGIN
        1 ctgtcgtcag tcaggtgaat aattttatcg ctcatctcga gcagctgcac ctcggccatg
       61 gctggttggg cagcgagtaa taacaatcca gcggctgccg taggcaatag gtatttcatt
      121 ttaaattcct cctaattaat tatctagaac taacactctc ccctgttgaa gctctttgtg
      181 acgggcaagc tcaggccctg atgggtgact tcgcaggcgt agactttgtg tttctcgtag
      241 tctgctttgc tcagcgtcag ggtgctgctg aggctgtagg tgctgtcctt gctgtcctgc
      301 tctgtgacac tctcctggga gttacccgat tggagggcgt tatccacctt ccactgtact
      361 ttggcctctc tgggatagaa gttattcagc aggcacacaa cagaggcagt tccagatttc
      421 aactgctcat cagatggcgg gaagatgaag acagatggtg cagccacagt tcgtttgagt
      481 tccaccttgg tcccttggcc gaaccacggt gagccaccat actgctgaca gtagtacact
      541 gcaaaatctt caggctccag tctgctgatg gtgagagtga agtctgtccc agacccactg
      601 ccactgaacc tgtctgggat gccagtggcc ctgctggatg taccatagat gaggagcctg
      661 ggagcctggc caggtttctg ctggtaccac ggtcaccgtc tccgcag
//
