LOCUS       sequence-111833-         686 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses aa1594–1670 of VWF A2 domain flanked by Venus and Cerulean
            and tagged with 7X His at C terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Dayananda KM, Gogia S, Neelamegham S
  TITLE     Escherichia coli-derived von Willebrand factor-A2 domain 
            fluorescence/Forster resonance energy transfer proteins that 
            quantify ADAMTS13 activity.
  JOURNAL   Anal Biochem. 2011 Mar 15;410(2):206-13. doi: 
            10.1016/j.ab.2010.12.005. Epub 2010 Dec 10.
  PUBMED    21146487
REFERENCE   2  (bases 1 to 686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ab.2010.12.005"; journalName: "Anal Biochem"; date: 
            "2011-03-15- 15"; volume: "410"; issue: "2"; pages: "206-13"
COMMENT     Sequence Label: pRSET-XS-VWF
FEATURES             Location/Qualifiers
     source          1..686
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(11..13,14..16,17..529)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(56..77)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(317..336)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     664..685
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 agatatacat atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
       61 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
      121 tgccacctac ggcaagctga ccctgaagct gatctgcacc accggcaagc tgcccgtgcc
      181 ctggcccacc ctcgtgacca ccctgggcta cggcctgcag tgcttcgccc gctaccccga
      241 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
      301 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
      361 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
      421 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca ccgccgacaa
      481 gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcggcgt
      541 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
      601 cgacaaccac tacctgagct accagtccaa gctgagcaaa gaccccaacg agaagcgcga
      661 tcacatggtc ctgctggagt tcgtga
//
