LOCUS       sequence-111836-         647 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses aa1594–1670 of VWF A2 domain flanked by Venus and Cerulean
            and tagged with 7X His at C terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Dayananda KM, Gogia S, Neelamegham S
  TITLE     Escherichia coli-derived von Willebrand factor-A2 domain 
            fluorescence/Forster resonance energy transfer proteins that 
            quantify ADAMTS13 activity.
  JOURNAL   Anal Biochem. 2011 Mar 15;410(2):206-13. doi: 
            10.1016/j.ab.2010.12.005. Epub 2010 Dec 10.
  PUBMED    21146487
REFERENCE   2  (bases 1 to 647)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ab.2010.12.005"; journalName: "Anal Biochem"; date: 
            "2011-03-15- 15"; volume: "410"; issue: "2"; pages: "206-13"
COMMENT     Sequence Label: pRSET-XS-VWF
FEATURES             Location/Qualifiers
     source          1..647
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(20..40)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     CDS             complement(41..61)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(65..316)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(107..128)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     456..475
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 agcagccgga tcaagcttaa tggtgatggt gatggtgatg gccctggaag tacaggtttt
       61 cgaacttgta cagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca
      121 ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag cttggactgg gtgctcaggt
      181 agtggttgtc gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt
      241 cggcgagctg cacgctgccg tcctcgatgt tgtggcggat cttgaagttg gccttgatgc
      301 cgttcttctg cttgtcggcg gtgatataga cgttgtcgct gatggcgttg tactccagct
      361 tgtgccccag gatgttgccg tcctccttga agtcgatgcc cttcagctcg atgcggttca
      421 ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga
      481 agaagatggt gcgctcctgg acgtagcctt cgggcatggc ggacttgaag aagtcgtgct
      541 gcttcatgtg gtcggggtag cgggcgaagc actgcacgcc ccaggtcagg gtggtcacga
      601 gggtgggcca gggcacgggc agcttgccgg tggtgcagat gaacttc
//
