LOCUS       sequence-111838-         687 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses aa 1594-1670 of A2 Domain of VWF protein, TEV cleavage 
            site and Venus followed by 7X His tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Gogia S, Lo CY, Neelamegham S
  TITLE     Detection of Plasma Protease Activity Using Microsphere-Cytometry 
            Assays with E. coli Derived Substrates: VWF Proteolysis by ADAMTS13.
  JOURNAL   PLoS One. 2015 May 18;10(5):e0126556. doi: 
            10.1371/journal.pone.0126556. eCollection 2015.
  PUBMED    25992814
REFERENCE   2  (bases 1 to 687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-05-18"; volume: "10(5):e0126556. doi"; pages: " 
            10.1371/journal.pone.0126556. eCollection 2015"
COMMENT     Sequence Label: pRSET-A2-77p-Venus
FEATURES             Location/Qualifiers
     source          1..687
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             254..274
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(320..341)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(581..600)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 agatatacat atgaaacagg gtgaccggga gcaggcgccc aacctggtct acatggtcac
       61 cggaaatcct gcctctgatg agatcaggag gctgcctgga gacatccagg tggtgcccat
      121 tggagtgggc cctaatgcca acgtgcagga gctggagagg attggctggc ccaatgcccc
      181 tatcctcatc caggactttg agacgctccc ccgagaggct cctgacctgg tgctgcagag
      241 gtgctgcttc gaagagaatc tatactttca aggcatggtg agcaagggcg aggagctgtt
      301 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      361 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agctgatctg
      421 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctgg gctacggcct
      481 gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
      541 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
      601 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
      661 cgacttcaag gaggacggca acatcct
//
