LOCUS       sequence-111841-         702 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses aa 1594-1670 of A2 Domain of VWF protein, TEV cleavage 
            site and Venus followed by 7X His tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Gogia S, Lo CY, Neelamegham S
  TITLE     Detection of Plasma Protease Activity Using Microsphere-Cytometry 
            Assays with E. coli Derived Substrates: VWF Proteolysis by ADAMTS13.
  JOURNAL   PLoS One. 2015 May 18;10(5):e0126556. doi: 
            10.1371/journal.pone.0126556. eCollection 2015.
  PUBMED    25992814
REFERENCE   2  (bases 1 to 702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-05-18"; volume: "10(5):e0126556. doi"; pages: " 
            10.1371/journal.pone.0126556. eCollection 2015"
COMMENT     Sequence Label: pRSET-A2-77p-Venus
FEATURES             Location/Qualifiers
     source          1..702
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(20..40)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     primer_bind     complement(74..95)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     423..442
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 agcagccgga tcaagcttag tgatggtggt ggtgatgatg ctcgtccatg cctagagtga
       61 tcccggcggc ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg ttggggtctt
      121 tgctcagctt ggactggtag ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc
      181 cgatgggggt gttctgctgg tagtggtcgg cgagctgcac gccgccgtcc tcgatgttgt
      241 ggcggatctt gaagttggcc ttgatgccgt tcttctgctt gtcggcggtg atatagacgt
      301 tgtggctgtt gtagttgtac tccagcttgt gccccaggat gttgccgtcc tccttgaagt
      361 cgatgccctt cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc
      421 gggtcttgta gttgccgtcg tccttgaaga agatggtgcg ctcctggacg tagccttcgg
      481 gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg gcgaagcact
      541 gcaggccgta gcccagggtg gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg
      601 tgcagatcag cttcagggtc agcttgccgt aggtggcatc gccctcgccc tcgccggaca
      661 cgctgaactt gtggccgttt acgtcgccgt ccagctcgac ca
//
