LOCUS       sequence-111851-       12292 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast dCas9-VP64 expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12292)
  AUTHORS   Zalatan JG, Lee ME, Almeida R, Gilbert LA, Whitehead EH, La Russa M,
            Tsai JC, Weissman JS, Dueber JE, Qi LS, Lim WA
  TITLE     Engineering Complex Synthetic Transcriptional Programs with CRISPR 
            RNA Scaffolds.
  JOURNAL   Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052.
  PUBMED    25533786
REFERENCE   2  (bases 1 to 12292)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052."
COMMENT     Sequence Label: pJZC519
FEATURES             Location/Qualifiers
     source          1..12292
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        276..683
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     promoter        2522..3188
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     3058..3081
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     CDS             7308..7328
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7344..7364
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7371..7520
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             7527..7547
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(8839..8858)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8840..8858)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8865..8882)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8865..8881)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8874..8896)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9022..9477
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9109..9128)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9319..9340
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9833..9852)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9952..9974
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10012..10030)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10115..10219
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10220..10288,10289..11080)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10438..10457)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11251..11839
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11740..11759
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11993..12010
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12127..12148
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12163..12168,12169..12186,12187..12193)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12201..12217
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12206..12228
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12225..12241
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12225..12241
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        12262..12280
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ggccggccat ttaaatgttt aaacggccac gctggccgag gagaacttct agtatatcta
       61 catacctaat attattgcct tattaaaaat ggaatcccaa caattacatc aaaatccaca
      121 ttctcttcaa aatcaattgt cctgtacttc cttgttcatg tgtgttcaaa aacgttatat
      181 ttataggata attatactct atttctcaac aagtaattgg ttgtttggcc gagcggtcta
      241 aggcgcctga ttcaagaaat atcttgaccg cagttaactg tgggaatact caggtatcgt
      301 aagatgcaag agttcgaatc tcttagcaac cattattttt ttcctcaaca taacgagaac
      361 acacaggggc gctatcgcac agaatcaaat tcgatgactg gaaatttttt gttaatttca
      421 gaggtcgcct gacgcatata cctttttcaa ctgaaaaatt gggagaaaaa ggaaaggtga
      481 gagcgccgga accggctttt catatagaat agagaagcgt tcatgactaa atgcttgcat
      541 cacaatactt gaagttgaca atattattta aggacctatt gttttttcca ataggtggtt
      601 agcaatcgtc ttactttcta acttttctta ccttttacat ttcagcaata tatatatata
      661 tatttcaagg atataccatt ctaatgtctg cccctaagtt accaatagat agcggtttaa
      721 tataaggttt caacaagaaa tattttgtga aaatcaaaag ttaatagttt cactgtcgaa
      781 aatatgaaag tttaagtttt cagttcatcc cattgtagtt atcaaatttc tgtgtttccc
      841 gtatatgtgt gctctgtatt catcgctatc ttgaaactgg ttatgatcgt tgcgcatgtg
      901 acctgtatcg ctcaaaagag cccggcaccg gtaccgggca aaagccccta gcaagctaaa
      961 atgattcaac tttcgtagta atttgttcaa ccggtcacag taaagtcaat gactaacttt
     1021 tgaatcgagg atgtatttcc aattgaaagc aatgtatata taaaccattg tgttgaagct
     1081 tgtcttacag ttttcttgta tcctcccatt gttcatatag tacttaaagt accataatcc
     1141 ttaaagaaca aacttgaaag agaatacact atacacaact aataaataaa gaataatcat
     1201 ggctgtgacc aagacaattg tagttctacc aggtgaccat gttggtcaag aaatcactga
     1261 agaggccatt aaagttttaa atgctattca ggaatgtcgt ccagacaagg tcaatttcaa
     1321 gtttgatcat catttgatcg gtggtgctgc aattgatgcc actggtgttc cattaccaga
     1381 cgaagctttg gaggcctcta agaaagctga tgccgtgctt ctgggtgctg ttggtggtcc
     1441 aaaatggggt actggcgctg tcagaccaga acaaggtctt ttgaaaatcc gtaaggagtt
     1501 gcaattgtat gctaatctaa gaccatgtaa ttttgcatct gattccttac tagatctatc
     1561 gccattgaag cctgaaattg caagaggtac agatttcgtc gttgttagag aactagtggg
     1621 tggtatttat ttcggggaga gaaaagaaga tgaaggtgat ggtgtcgcct gggatagcga
     1681 aaagtattct gtgcccgaag ttcaaagaat cactagaatg gctgctttca tggctctgca
     1741 acacaaccca cctttaccta tttggtctct agataaagcc aacgttctag catcatcaag
     1801 attatggaga aaaacagtgg aagagacgat taagaatgaa ttcccacaat tgactgttca
     1861 acatcaattg attgattccg ctgctatgat cttggttaag aacccaactc atctaaatgg
     1921 tattattatc acaaacaata tgtttggtga tattatctcc gatgaagcct ccgtgatccc
     1981 aggttcgtta ggattacttc catctgcctc cttggcatct cttccagata agaatacggc
     2041 atttggttta tatgagccat gtcatggctc tgctccagac ttaccaaagg gaaaggttaa
     2101 ccccgtagca acaatcctat ctgctgctat gatgttgaag ttgtctctgg acctgttcga
     2161 agaaggtgaa attatagaac aagcagtcaa gaaagtgttg gattctggta tcagaacagc
     2221 ggatctaaag ggtaccaact ctactactga ggtcggtgat gcagtagcga aagctgtcag
     2281 ggaactatta gcttagactt agtagatgta cagttgcata cataagtctg aacagaaaga
     2341 agtaaatatc atatctagtt cggtttagaa atcagtgact atattggttt tgaccagatc
     2401 gcaatccttg caagcattta gtaatgacta tcaaaccggt gaatcatctg ataaggctat
     2461 attagcttgt gcattcgcat gtatcgggaa acgaacggta ccgggcccca gttcgagttt
     2521 atcattatca atactgccat ttcaaagaat acgtaaataa ttaatagtag tgattttcct
     2581 aactttattt agtcaaaaaa ttagcctttt aattctgctg taacccgtac atgcccaaaa
     2641 tagggggcgg gttacacaga atatataaca tcgtaggtgt ctgggtgaac agtttattcc
     2701 tggcatccac taaatataat ggagcccgct ttttaagctg gcatccagaa aaaaaaagaa
     2761 tcccagcacc aaaatattgt tttcttcacc aaccatcagt tcataggtcc attctcttag
     2821 cgcaactaca gagaacaggg gcacaaacag gcaaaaaacg ggcacaacct caatggagtg
     2881 atgcaacctg cctggagtaa atgatgacac aaggcaattg acccacgcat gtatctatct
     2941 cattttctta caccttctat taccttctgc tctctctgat ttggaaaaag ctgaaaaaaa
     3001 aggttgaaac cagttccctg aaattattcc cctacttgac taataagtat ataaagacgg
     3061 taggtattga ttgtaattct gtaaatctat ttcttaaact tcttaaattc tacttttata
     3121 gttagtcttt tttttagttt taaaacacca agaacttagt ttcgaataaa cacacataaa
     3181 caaacaaaat gctcgaggac aagaagtatt ctatcggact ggccatcggg actaatagcg
     3241 tcgggtgggc cgtgatcact gacgagtaca aggtgccctc taagaagttc aaggtgctcg
     3301 ggaacaccga ccggcattcc atcaagaaaa atctgatcgg agctctcctc tttgattcag
     3361 gggagaccgc tgaagcaacc cgcctcaagc ggactgctag acggcggtac accaggagga
     3421 agaaccggat ttgttacctt caagagatat tctccaacga aatggcaaag gtcgacgaca
     3481 gcttcttcca taggctggaa gaatcattcc tcgtggaaga ggataagaag catgaacggc
     3541 atcccatctt cggtaatatc gtcgacgagg tggcctatca cgagaaatac ccaaccatct
     3601 accatcttcg caaaaagctg gtggactcaa ccgacaaggc agacctccgg cttatctacc
     3661 tggccctggc ccacatgatc aagttcagag gccacttcct gatcgagggc gacctcaatc
     3721 ctgacaatag cgatgtggat aaactgttca tccagctggt gcagacttac aaccagctct
     3781 ttgaagagaa ccccatcaat gcaagcggag tcgatgccaa ggccattctg tcagcccggc
     3841 tgtcaaagag ccgcagactt gagaatctta tcgctcagct gccgggtgaa aagaaaaatg
     3901 gactgttcgg gaacctgatt gctctttcac ttgggctgac tcccaatttc aagtctaatt
     3961 tcgacctggc agaggatgcc aagctgcaac tgtccaagga cacctatgat gacgatctcg
     4021 acaacctcct ggcccagatc ggtgaccaat acgccgacct tttccttgct gctaagaatc
     4081 tttctgacgc catcctgctg tctgacattc tccgcgtgaa cactgaaatc accaaggccc
     4141 ctctttcagc ttcaatgatt aagcggtatg atgagcacca ccaggacctg accctgctta
     4201 aggcactcgt ccggcagcag cttccggaga agtacaagga aatcttcttt gaccagtcaa
     4261 agaatggata cgccggctac atcgacggag gtgcctccca agaggaattt tataagttta
     4321 tcaaacctat ccttgagaag atggacggca ccgaagagct cctcgtgaaa ctgaatcggg
     4381 aggatctgct gcggaagcag cgcactttcg acaatgggag cattccccac cagatccatc
     4441 ttggggagct tcacgccatc cttcggcgcc aagaggactt ctaccccttt cttaaggaca
     4501 acagggagaa gattgagaaa attctcactt tccgcatccc ctactacgtg ggacccctcg
     4561 ccagaggaaa tagccggttt gcttggatga ccagaaagtc agaagaaact atcactccct
     4621 ggaacttcga agaggtggtg gacaagggag ccagcgctca gtcattcatc gaacggatga
     4681 ctaacttcga taagaacctc cccaatgaga aggtcctgcc gaaacattcc ctgctctacg
     4741 agtactttac cgtgtacaac gagctgacca aggtgaaata tgtcaccgaa gggatgagga
     4801 agcccgcatt cctgtcaggc gaacaaaaga aggcaattgt ggaccttctg ttcaagacca
     4861 atagaaaggt gaccgtgaag cagctgaagg aggactattt caagaaaatt gaatgcttcg
     4921 actctgtgga gattagcggg gtcgaagatc ggttcaacgc aagcctgggt acctaccatg
     4981 atctgcttaa gatcatcaag gacaaggatt ttctggacaa tgaggagaaa gaggacatcc
     5041 ttgaggacat tgtcctgact ctcactctgt tcgaggaccg ggaaatgatc gaggagaggc
     5101 ttaagaccta cgcccatctg ttcgacgata aagtgatgaa gcaacttaaa cggagaagat
     5161 ataccggatg gggacgcctt agccgcaaac tcatcaacgg aatccgggac aaacagagcg
     5221 gaaagaccat tcttgatttc cttaagagcg acggattcgc taatcgcaac ttcatgcaac
     5281 ttatccatga tgattccctg acctttaagg aggacatcca gaaggcccaa gtgtctggac
     5341 aaggtgactc actgcacgag catatcgcaa atctggctgg ttcacccgct attaagaagg
     5401 gtattctcca gaccgtgaaa gtcgtggacg agctggtcaa ggtgatgggt cgccataaac
     5461 cagagaacat tgtcatcgag atggccaggg aaaaccagac tacccagaag ggacagaaga
     5521 acagcaggga gcggatgaaa agaattgagg aagggattaa ggagctcggg tcacagatcc
     5581 ttaaagagca cccggtggaa aacacccagc ttcagaatga gaagctctat ctgtactacc
     5641 ttcaaaatgg acgcgatatg tatgtggacc aagagcttga tatcaacagg ctctcagact
     5701 acgacgtgga cgccatcgtc cctcagagct tcctcaaaga cgactcaatt gacaataagg
     5761 tgctgactcg ctcagacaag aaccggggaa agtcagataa cgtgccctca gaggaagtcg
     5821 tgaaaaagat gaagaactat tggcgccagc ttctgaacgc aaagctgatc actcagcgga
     5881 agttcgacaa tctcactaag gctgagaggg gcggactgag cgaactggac aaagcaggat
     5941 tcattaaacg gcaacttgtg gagactcggc agattactaa acatgtagcc caaatccttg
     6001 actcacgcat gaataccaag tacgacgaaa acgacaaact tatccgcgag gtgaaggtga
     6061 ttaccctgaa gtccaagctg gtcagcgatt tcagaaagga ctttcaattc tacaaagtgc
     6121 gggagatcaa taactatcat catgctcatg acgcatatct gaatgccgtg gtgggaaccg
     6181 ccctgatcaa gaagtaccca aagctggaaa gcgagttcgt gtacggagac tacaaggtct
     6241 acgacgtgcg caagatgatt gccaaatctg agcaggagat cggaaaggcc accgcaaagt
     6301 acttcttcta cagcaacatc atgaatttct tcaagaccga aatcaccctt gcaaacggtg
     6361 agatccggaa gaggccgctc atcgagacta atggggagac tggcgaaatc gtgtgggaca
     6421 agggcagaga tttcgctacc gtgcgcaaag tgctttctat gcctcaagtg aacatcgtga
     6481 agaaaaccga ggtgcaaacc ggaggctttt ctaaggaatc aatcctcccc aagcgcaact
     6541 ccgacaagct cattgcaagg aagaaggatt gggaccctaa gaagtacggc ggattcgatt
     6601 caccaactgt ggcttattct gtcctggtcg tggctaaggt ggaaaaagga aagtctaaga
     6661 agctcaagag cgtgaaggaa ctgctgggta tcaccattat ggagcgcagc tccttcgaga
     6721 agaacccaat tgactttctc gaagccaaag gttacaagga agtcaagaag gaccttatca
     6781 tcaagctccc aaagtatagc ctgttcgaac tggagaatgg gcggaagcgg atgctcgcct
     6841 ccgctggcga acttcagaag ggtaatgagc tggctctccc ctccaagtac gtgaatttcc
     6901 tctaccttgc aagccattac gagaagctga aggggagccc cgaggacaac gagcaaaagc
     6961 aactgtttgt ggagcagcat aagcattatc tggacgagat cattgagcag atttccgagt
     7021 tttctaaacg cgtcattctc gctgatgcca acctcgataa agtccttagc gcatacaata
     7081 agcacagaga caaaccaatt cgggagcagg ctgagaatat catccacctg ttcaccctca
     7141 ccaatcttgg tgcccctgcc gcattcaagt acttcgacac caccatcgac cggaaacgct
     7201 atacctccac caaagaagtg ctggacgcca ccctcatcca ccagagcatc accggacttt
     7261 acgaaactcg gattgacctc tcacagctcg gaggggatga gggagctccc aagaaaaagc
     7321 gcaaggtagg tagttccgga tctccgaaaa agaaacgcaa agttggtagt gatgctttag
     7381 acgattttga cttagatatg cttggttcag acgcgttaga cgacttcgac ctagacatgt
     7441 taggctcaga tgcattggac gacttcgatt tagatatgtt gggctccgat gccctagatg
     7501 actttgatct agatatgcta ggtagtccca aaaagaagag gaaagtgtag gcggccgcca
     7561 ccgcggtgga gctctaagca aatagctaaa ttatatacga attaatatta tgattaagtg
     7621 tttacgtgag tgcgatattt ttattactat cttatacagt tgtatatact ctataaaatg
     7681 agttgtctat taattaacgc gatgaatgct ttctgggttt acctctccaa caactctagt
     7741 ttacttctca atacattcaa ttgtatttga tttgtcaata cttcatcatt aatcaattct
     7801 atagttttgt ttttctcgtt tatttccaaa tttaatgcat caattttatt attcaatttg
     7861 tcgttgattt tggttaatga ttttatggtt tgatctctgg cattgattgt ttgtgttagt
     7921 ttttcattat tgataattaa attatttaag ttagttatca actcggtgtt ttcaagtttc
     7981 aagttttcaa tttctttaga gtttattaga tttgtcaaag tttctgaatt gcttgattgg
     8041 tcctgtagaa gagtatttgt tgttgtggat aattgattca atttttgaga caattgctgg
     8101 aaggcgttga aatatctagc atcaatctca tggttttttt cccgagagtc tcgtagattc
     8161 aattgtttta atatatcttg ggaccactct tgatttgaac tcatggaaat taaactgggt
     8221 gttgtgttgt ggtgtaatga ttgtaccccc tttgcttata attgtgtggc caccgaagtc
     8281 ggtgatgctg tcgccgaaga agttaagaaa atccttgctt aaaaagattc tcttttttta
     8341 tgatatttgt acataaactt tataaatgaa attcataata gaaacgacac gaaattacaa
     8401 aatggaatat gttcataggg tagacgaaac tatatacgca atctacatac atttatcaag
     8461 aaggagaaaa aggaggatgt aaaggaatac aggtaagcaa attgatacta atggctcaac
     8521 gtgataagga aaaagaattg cactttaaca ttaatattga caaggaggag ggcaccacac
     8581 aaaaagttag gtgtaacaga aaatcatgaa actatgattc ctaatttata tattggagga
     8641 ttttctctaa aaaaaaaaaa atacaacaaa taaaaaacac tcaatgacct gaccatttga
     8701 tggagtttaa gtcaatacct ccttgaacca tttcccataa tggtgaaagt tccctcaaga
     8761 attttactct gtcagaaacg gccttaacga cgtaggccgg ccatttaaat gtttaaacgg
     8821 ccacgctggc ccaattcgcc ctatagtgag tcgtattaca attcactggc cgtcgtttta
     8881 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     8941 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     9001 cgcagcctga atggcgaatg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     9061 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     9121 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     9181 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     9241 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     9301 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     9361 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     9421 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttcct
     9481 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatag ggtaataact
     9541 gatataatta aattgaagct ctaatttgtg agtttagtat taggatctct acccttggcg
     9601 aaaagtcctc tgccaacaat gatgatatct gatccaccac ttacaacttc gtcgacggtt
     9661 ctgtactgct gacccaatat gcgtatatat accaatctaa gtctgtgctc cttccttcgt
     9721 tcttccttct gttcggagat taccgaatca aaaaaatttc aaggaaaccg aaatcaaaaa
     9781 aaagaataaa aaaaaaatga tgaattgaaa aggtggtatg gtgcactctc agtacaatct
     9841 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
     9901 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     9961 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
    10021 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcggcgcgcc
    10081 gaggacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
    10141 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
    10201 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
    10261 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
    10321 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
    10381 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
    10441 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
    10501 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
    10561 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
    10621 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
    10681 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
    10741 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
    10801 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
    10861 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
    10921 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
    10981 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
    11041 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
    11101 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
    11161 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
    11221 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
    11281 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
    11341 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
    11401 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
    11461 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
    11521 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
    11581 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
    11641 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
    11701 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
    11761 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
    11821 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
    11881 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
    11941 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
    12001 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
    12061 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
    12121 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
    12181 ggctcctatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
    12241 ccatgattac gccaagctcg gaattaaccc tcactaaagg gaacaaaagc tg
//
