LOCUS       sequence-111882-         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Part Plasmid Entry Vector to be used in the Dueber YTK system..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK001
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             259..270
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
       61 ctttcctgcg ttatcccctg attctgtgga taaccgtagt cggcgagacg gaaagtgaaa
      121 cgtgatttca tgcgtcattt tgaacatttt gtaaatctta tttaataatg tgtgcggcaa
      181 ttcacattta atttatgaat gttttcttaa catcgcggca actcaagaaa cggcaggttc
      241 ggatcttagc tactagagaa agaggagaaa tactagatgc gtaaaggcga agagctgttc
      301 actggtgtcg tccctattct ggtggaactg gatggtgatg tcaacggtca taagttttcc
      361 gtgcgtggcg agggtgaagg tgacgcaact aatggtaaac tgacgctgaa gttcatctgt
      421 actactggta aactgccggt tccttggccg actctggtaa cgacgctgac ttatggtgtt
      481 cagtgctttg ctcgttatcc ggaccatatg aagcagcatg acttcttcaa gtccgccatg
      541 ccggaaggct atgtgcagga acgcacgatt tcctttaagg atgacggcac gtacaaaacg
      601 cgtgcggaag tgaaatttga aggcgatacc ctggtaaacc gcattgagct gaaaggcatt
      661 gactttaaag aggacggcaa tatcctgggc cataagctgg aatacaattt taacagccac
      721 aatgtttaca tcaccgccga taaacaaaaa aatggcatta aagcgaattt taaaattcgc
      781 cacaacgtgg aggatggcag cgtgcagctg gctga
//
