LOCUS       sequence-111894-         756 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses human codon-optimised TEV protease.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Oberst A, Pop C, Tremblay AG, Blais V, Denault JB, Salvesen GS, 
            Green DR
  TITLE     Inducible dimerization and inducible cleavage reveal a requirement 
            for both processes in caspase-8 activation.
  JOURNAL   J Biol Chem. 2010 May 28;285(22):16632-42. doi: 
            10.1074/jbc.M109.095083. Epub 2010 Mar 22.
  PUBMED    20308068
REFERENCE   2  (bases 1 to 756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M109.095083"; journalName: "J Biol Chem"; date: 
            "2010-05-28- 28"; volume: "285"; issue: "22"; pages: "16632-42"
COMMENT     Sequence Label: pcDNA3.1-V5-hTEV
FEATURES             Location/Qualifiers
     source          1..756
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..45
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             46..753
                     /codon_start=1
                     /product="tobacco etch virus protease (Kapust et al., 
                     2001)"
                     /label=TEV protease
                     /translation="GESLFKGPRDYNPISSTICHLTNESDGHTTSLYGIGFGPFIITNK
                     HLFRRNNGTLLVQSLHGVFKVKNTTTLQQHLIDGRDMIIIRMPKDFPPFPQKLKFREPQ
                     REERICLVTTNFQTKSMSSMVSDTSCTFPSSDGIFWKHWIQTKDGQCGSPLVSTRDGFI
                     VGIHSASNFTNTNNYFTSVPKNFMELLTNQEAQQWVSGWRLNADSVLWGGHKVFMVKPE
                     EPFQPVKEATQLMN"
ORIGIN
        1 atgggcaaac ctattcctaa tccactgctc ggactcgaca gcacaggcga gagcctcttc
       61 aagggaccca gggactacaa ccccattagc agcaccatct gccacctcac caatgagtcc
      121 gacggccata ccaccagcct gtatggaatc ggctttggac ctttcatcat caccaacaag
      181 catctgtttc ggcggaacaa cggcaccctg ctggtgcaga gcctgcatgg cgtgttcaag
      241 gtcaagaaca caactaccct ccagcagcac ctgatcgatg gaagagacat gatcatcatc
      301 cggatgccaa aagactttcc accttttccc caaaaactca aattccggga gcctcagaga
      361 gaagagcgga tctgtctggt gaccacaaat ttccagacaa aaagcatgag cagcatggtg
      421 tccgatacaa gctgcacatt cccatccagc gatggcatct tctggaaaca ttggattcag
      481 acaaaagatg gccagtgcgg aagcccactc gtctccacta gagacgggtt catcgtgggg
      541 attcacagcg ctagcaattt tacaaatacc aataattact ttacatccgt cccaaagaac
      601 ttcatggagc tgctgacaaa ccaggaagca cagcagtggg tgagcggctg gaggctgaac
      661 gccgattccg tcctgtgggg cggccacaag gtcttcatgg tgaagcccga agagcctttc
      721 cagccagtga aggaggccac tcaactgatg aactaa
//
