LOCUS       sequence-111896-        1085 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  harbours marker gene LEU2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Mulleder M, Campbell K, Matsarskaia O, Eckerstorfer F, Ralser M
  TITLE     Saccharomyces cerevisiae single-copy plasmids for auxotrophy 
            compensation, multiple marker selection, and for designing 
            metabolically cooperating communities.
  JOURNAL   F1000Res. 2016 Sep 20;5:2351. eCollection 2016.
  PUBMED    27830062
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "F1000Res.";
            date: "2016-09-20"; volume: "5"; pages: "2351. eCollection 2016"
COMMENT     Sequence Label: pL
FEATURES             Location/Qualifiers
     source          1..1085
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(217..624)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     primer_bind     948..970
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1008..1026)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 aacggcatca gccttcttgg aggcttccag cgcctcatct ggaagtggaa cacctgtagc
       61 atcgatagca gcaccaccaa ttaaatgatt ttcgaaatcg aacttgacat tggaacgaac
      121 atcagaaata gctttaagaa ccttaatggc ttcggctgtg atttcttgac caacgtggtc
      181 acctggcaaa acgacgatct tcttaggggc agacattaga atggtatatc cttgaaatat
      241 atatatatat attgctgaaa tgtaaaaggt aagaaaagtt agaaagtaag acgattgcta
      301 accacctatt ggaaaaaaca ataggtcctt aaataatatt gtcaacttca agtattgtga
      361 tgcaagcatt tagtcatgaa cgcttctcta ttctatatga aaagccggtt ccggcgctct
      421 cacctttcct ttttctccca atttttcagt tgaaaaaggt atatgcgtca ggcgacctct
      481 gaaattaaca aaaaatttcc agtcatcgaa tttgattctg tgcgatagcg cccctgtgtg
      541 ttctcgttat gttgaggaaa aaaataatgg ttgctaagag attcgaactc ttgcatctta
      601 cgatacctga gtattcccac agttaactgc ggtcaagata tttcttgaat caggcgcctt
      661 agaccgctcg gccaaacaac caattacttg ttgagaaata gagtataatt atcctataaa
      721 tataacgttt ttgaacacac atgaacaagg aagtacagga caattgattt tgaagagaat
      781 gtggattttg atgtaattgt tgggattcca tttttaataa ggcaataata ttaggtatgt
      841 agatatacta gaagttctcc tcgatggcgc gccgccaaca cccgctgacg cgccctgacg
      901 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
      961 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
     1021 cctattttta taggttaatg tcatgataat aatggtttct taggtccttt tcatcacgtg
     1081 ctata
//
