LOCUS       sequence-111923-       10431 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of eIF4G-MS2 fusion. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10431)
  AUTHORS   Fatscher T, Boehm V, Weiche B, Gehring NH
  TITLE     The interaction of cytoplasmic poly(A)-binding protein with 
            eukaryotic initiation factor 4G suppresses nonsense-mediated mRNA 
            decay.
  JOURNAL   RNA. 2014 Oct;20(10):1579-92. doi: 10.1261/rna.044933.114. Epub 2014
            Aug 21.
  PUBMED    25147240
REFERENCE   2  (bases 1 to 10431)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.044933.114"; journalName: "RNA"; date: "2014-10"; 
            volume: "20"; issue: "10"; pages: "1579-92"
COMMENT     Sequence Label: pCI-MS2V5-eIF4G delN83
FEATURES             Location/Qualifiers
     source          1..10431
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     680..700
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     726..750
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          890..1022
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1005..1024
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1067..1085
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1097..1483
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2
                     /note="binds to a specific stem-loop structure in the viral
                     RNA (Peabody, 1993)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQNRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             1490..1531
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     polyA_signal    6135..6256
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6146..6165)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6200..6219
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      6442..6897
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6529..6548)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6739..6760
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7014..7371
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7014..7205
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7037..7057)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7222..7357
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7422..8216
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7476..7495)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8086..8105
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8280..8328
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(8369..8388)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8488..8510
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8548..8566)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8634..8738
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8739..8807,8808..9599)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8957..8976)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9770..10358
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10259..10278
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg
      661 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac
      781 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt
      841 gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa
      901 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact
      961 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac
     1021 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact
     1081 ataggctagc accatggctt ctaactttac tcagttcgtt ctcgtggaca atggcggaac
     1141 tggcgacgtg actgtcgccc caagcaactt cgctaacggg gtcgctgaat ggatcagctc
     1201 taactcgcgt tcacaggctt acaaagtaac ctgtagcgtt cgtcagagct ctgcgcagaa
     1261 tcgcaaatac accatcaaag tcgaggtgcc taaagtggca acccagactg ttggtggtgt
     1321 agagcttcct gtagccgcat ggcgttcgta cttaaatatg gaactaacca ttccaatttt
     1381 cgccacgaac tccgactgcg agcttattgt taaggcaatg caaggtctcc taaaagatgg
     1441 aaacccgatt ccctcagcaa tcgcagcaaa ctccggcatc tacggcggtg gcaagcccat
     1501 ccctaaccca ctgctgggcc tggacagcac cctcgaggga tcccaagtaa tgatgatccc
     1561 ttcccagatc tcctacccag cctcccaggg ggcctactac atccctggac aggggcgttc
     1621 cacatacgtt gtcccgacac agcagtaccc tgtgcagcca ggagccccag gcttctatcc
     1681 aggtgcaagc cctacagaat ttgggaccta cgctggcgcc tactatccag cccaaggggt
     1741 gcagcagttt cccactggcg tggcccccgc cccagttttg atgaaccagc caccccagat
     1801 tgctcccaag agggagcgta agacgatccg aattcgagat ccaaaccaag gaggaaagga
     1861 tatcacagag gagatcatgt ctggggcccg cactgcctcc acacccaccc ctccccagac
     1921 gggaggcggt ctggagcctc aagctaatgg ggagacgccc caggttgctg tcattgtccg
     1981 gccagatgac cggtcacagg gagcaatcat tgctgaccgg ccagggctgc ctggcccaga
     2041 gcatagccct tcagaatccc agccttcgtc gccttctccg accccatcac catccccagt
     2101 cttggaaccg gggtctgagc ctaatctcgc agtcctctct attcctgggg acactatgac
     2161 aactatacaa atgtctgtag aagaattaac ccccatctcc cgtgaaactg gggagccata
     2221 tcgcctctct ccagaaccca ctcctctcgc cgaacccata ctggaagtag aagtgacact
     2281 tagcaaaccg gttccagaat ctgagttttc ttccagtcct ctccaggctc ccaccccttt
     2341 ggcatctcac acagtggaaa ttcatgagcc taatggcatg gtcccatctg aagatctgga
     2401 accagaggtg gagtcaagcc cagagcttgc tcctccccca gcttgcccct ccgaatcccc
     2461 tgtgcccatt gctccaactg cccaacctga ggaactgctc aacggagccc cctcgccacc
     2521 agctgtggac ttaagcccag tcagtgagcc agaggagcag gccaaggagg tgacagcatc
     2581 agtggcgccc cccaccatcc cctctgctac tccagctacg gctccttcag ctacttcccc
     2641 agctcaggag gaggaaatgg aagaagaaga agaagaggaa gaaggagaag caggagaagc
     2701 aggagaagct gagagtgaga aaggaggaga ggaactgctc cccccagaga gtacccctat
     2761 tccagccaac ttgtctcaga atttggaggc agcagcagcc actcaagtgg cagtatctgt
     2821 gccaaagagg agacggaaaa ttaaggagct aaataagaag gaggctgttg gagaccttct
     2881 ggatgccttc aaggaggcga acccggcagt accagaggtg gaaaatcagc ctcctgcagg
     2941 cagcaatcca ggcccagagt ctgagggcag tggtgtgccc ccacgtcctg aggaagcaga
     3001 tgagacctgg gactcaaagg aagacaaaat tcacaatgct gagaacatcc agcccgggga
     3061 acagaagtat gaatataagt cagatcagtg gaagcctcta aacctagagg agaaaaaacg
     3121 ttacgaccgt gagttcctgc ttggttttca gttcatcttt gccagtatgc agaagccaga
     3181 gggattgcca catatcagtg acgtggtgct ggacaaggcc aataaaacac cactgcggcc
     3241 actggatccc actagactac aaggcataaa ttgtggccca gacttcactc catcctttgc
     3301 caaccttggc cggacaaccc ttagcacccg tgggccccca aggggtgggc caggtgggga
     3361 gctgccccgt gggccggctg gcctgggacc ccggcgctct cagcagggac cccgaaaaga
     3421 accacgcaag atcattgcca cagtgttaat gaccgaagat ataaaactga acaaagcaga
     3481 gaaagcctgg aaacccagca gcaagcggac ggcggctgat aaggatcgag gggaagaaga
     3541 tgctgatggc agcaaaaccc aggacctatt ccgcagggtg cgctccatcc tgaataaact
     3601 gacaccccag atgttccagc agctgatgaa gcaagtgacg cagctggcca tcgacaccga
     3661 ggaacgcctc aaaggggtca ttgacctcat ttttgagaag gccatttcag agcccaactt
     3721 ctctgtggcc tatgccaaca tgtgccgctg cctcatggcg ctgaaagtgc ccactacgga
     3781 aaagccaaca gtgactgtga acttccgaaa gctgttgttg aatcgatgtc agaaggagtt
     3841 tgagaaagac aaagatgatg atgaggtttt tgagaagaag caaaaagaga tggatgaagc
     3901 tgctacggca gaggaacgag gacgcctgaa ggaagagctg gaagaggctc gggacatagc
     3961 ccggcggcgc tctttaggga atatcaagtt tattggagag ttgttcaaac tgaagatgtt
     4021 aacagaggca ataatgcatg actgtgtggt caaactgctt aagaaccatg atgaagagtc
     4081 ccttgagtgc ctttgtcgtc tgctcaccac cattggcaaa gacctggact ttgaaaaagc
     4141 caagccccga atggatcagt atttcaacca gatggaaaaa atcattaaag aaaagaagac
     4201 gtcatcccgc atccgcttta tgctgcagga cgtgctggat ctgcgaggga gcaattgggt
     4261 gccacgccga ggggatcagg gtcccaagac cattgaccag atccataagg aggctgagat
     4321 ggaagaacat cgagagcaca tcaaagtgca gcagctcatg gccaagggca gtgacaagcg
     4381 tcggggcggt cctccaggcc ctcccatcag ccgtggactt ccccttgtgg atgatggtgg
     4441 ctggaacaca gttcccatca gcaaaggtag ccgccccatt gacacctcac gactcaccaa
     4501 gatcaccaag cctggctcca tcgattctaa caaccagctc tttgcacctg gagggcgact
     4561 gagctggggc aagggcagca gcggaggctc aggagccaag ccctcagacg cagcatcaga
     4621 agctgctcgc ccagctacta gtactttgaa tcgcttctca gcccttcaac aagcggtacc
     4681 cacagaaagc acagataata gacgtgtggt gcagaggagt agcttgagcc gagaacgagg
     4741 cgagaaagct ggagaccgag gagaccgcct agagcggagt gaacggggag gggaccgtgg
     4801 ggaccggctt gatcgtgcgc ggacaccagc taccaagcgg agcttcagca aggaagtgga
     4861 ggagcggagt agagaacggc cctcccagcc tgaggggctg cgcaaggcag ctagcctcac
     4921 ggaggatcgg gaccgtgggc gggatgccgt gaagcgagaa gctgccctac ccccagtgag
     4981 ccccctgaag gcggctctct ctgaggagga gttagagaag aaatccaagg ctatcattga
     5041 ggaatatctc catctcaatg acatgaaaga ggcagtccag tgcgtgcagg agctggcctc
     5101 accctccttg ctcttcatct ttgtacggca tggtgtcgag tctacgctgg agcgcagtgc
     5161 cattgctcgt gagcatatgg ggcagctgct gcaccagctg ctctgtgctg ggcatctgtc
     5221 tactgctcag tactaccaag ggttgtatga aatcttggaa ttggctgagg acatggaaat
     5281 tgacatcccc cacgtgtggc tctacctagc ggaactggta acacccattc tgcaggaagg
     5341 tggggtgccc atgggggagc tgttcaggga gattacaaag cctctgagac cgttgggcaa
     5401 agctgcttcc ctgttgctgg agatcctggg cctcctgtgc aaaagcatgg gtcctaaaaa
     5461 ggtggggacg ctgtggcgag aagccgggct tagctggaag gaatttctac ctgaaggcca
     5521 ggacattggt gcattcgtcg ctgaacagaa ggtggagtat accctgggag aggagtcgga
     5581 agcccctggc cagagggcac tcccctccga ggagctgaac aggcagctgg agaagctgct
     5641 gaaggagggc agcagtaacc agcgggtgtt cgactggata gaggccaacc tgagtgagca
     5701 gcagatagta tccaacacgt tagttcgagc cctcatgacg gctgtctgct attctgcaat
     5761 tatttttgag actcccctcc gagtggacgt tgcagtgctg aaagcgcgag cgaagctgct
     5821 gcagaaatac ctgtgtgacg agcagaagga gctacaggcg ctctacgccc tccaggccct
     5881 tgtagtgacc ttagaacagc ctcccaacct gctgcggatg ttctttgacg cactgtatga
     5941 cgaggacgtg gtgaaggagg atgccttcta cagttgggag agtagcaagg accccgctga
     6001 gcagcagggc aagggtgtgg cccttaaatc tgtcacagcc ttcttcaagt ggctccgtga
     6061 agcagaggag gagtctgacc acaactaagc ggccgcttcc ctttagtgag ggttaatgct
     6121 tcgagcagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
     6181 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     6241 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
     6301 gatgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa tccgataagg
     6361 atcgatccgg gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     6421 cgcagcctga atggcgaatg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     6481 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     6541 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     6601 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     6661 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     6721 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     6781 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     6841 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttcct
     6901 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac gcggatctgc
     6961 gcagcaccat ggcctgaaat aacctctgaa agaggaactt ggttaggtac cttctgaggc
     7021 ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     7081 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     7141 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     7201 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     7261 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     7321 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttgat
     7381 tcttctgaca caacagtctc gaacttaagg ctagagccac catgattgaa caagatggat
     7441 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     7501 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     7561 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc
     7621 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     7681 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     7741 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     7801 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     7861 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     7921 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga
     7981 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     8041 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     8101 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     8161 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg
     8221 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gatggccgca
     8281 ataaaatatc tttattttca ttacatctgt gtgttggttt tttgtgtgaa tcgatagcga
     8341 taaggatccg cgtatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     8401 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     8461 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     8521 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     8581 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     8641 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     8701 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     8761 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     8821 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     8881 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     8941 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     9001 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     9061 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     9121 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     9181 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     9241 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     9301 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     9361 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     9421 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     9481 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     9541 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     9601 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     9661 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     9721 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     9781 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     9841 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     9901 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     9961 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
    10021 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
    10081 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
    10141 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
    10201 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
    10261 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
    10321 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
    10381 tacggttcct ggccttttgc tggccttttg ctcacatggc tcgacagatc t
//
