LOCUS       sequence-111926-         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral Gateway destination vector, expression, CMV 
            promoter, Hygro.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pLenti CMV Hygro DEST (w117-1)
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(85..91,92..109,110..115))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(140..264)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(348..372)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(369..572)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(398..418)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg
       61 acggatccta actcaaaatc cacacattat acgagccgga agcataaagt gtaaagcctg
      121 gggtgcctaa tgcggccgcc atagtgactg gatatgttgt gttttacagt attatgtagt
      181 ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg
      241 ttcagctttt ttgtacaaac ttgttgatat ctgcagaatt ccaccacact ggactagagt
      301 cggtgtcttc tatggaggtc aaaacagcgt ggatggcgtc tccaggcgat ctgacggttc
      361 actaaacgag ctctgcttat atagacctcc caccgtacac gcctaccgcc catttgcgtc
      421 aatggggcgg agttgttacg acattttgga aagtcccgtt gattttggtg ccaaaacaaa
      481 ctcccattga cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca
      541 cgcccattga tgtactgcca aaaccgcatc accatggtaa tagcgatgac taatacgtag
      601 atgtactgcc aagtaggaaa gtcccataag gtcatgtact gggcataatg ccaggcgggc
      661 catttaccgt cattgacgtc aatagggggc gtacttggca tatgatacac ttgatgtact
      721 gccaagtggg cagtttaccg taaatactcc acccattgac gtcaatggaa agtccctatt
      781 ggcgttacta tgggaacata cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt
      841 cagccagg
//
