LOCUS       sequence-111929-        1135 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  harbours marker genes HIS3, LEU2, URA3 and LYS2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Mulleder M, Campbell K, Matsarskaia O, Eckerstorfer F, Ralser M
  TITLE     Saccharomyces cerevisiae single-copy plasmids for auxotrophy 
            compensation, multiple marker selection, and for designing 
            metabolically cooperating communities.
  JOURNAL   F1000Res. 2016 Sep 20;5:2351. eCollection 2016.
  PUBMED    27830062
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "F1000Res.";
            date: "2016-09-20"; volume: "5"; pages: "2351. eCollection 2016"
COMMENT     Sequence Label: pHLUK
FEATURES             Location/Qualifiers
     source          1..1135
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(221..628)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
ORIGIN
        1 acaaaacggc atcagccttc ttggaggctt ccagcgcctc atctggaagt ggaacacctg
       61 tagcatcgat agcagcacca ccaattaaat gattttcgaa atcgaacttg acattggaac
      121 gaacatcaga aatagcttta agaaccttaa tggcttcggc tgtgatttct tgaccaacgt
      181 ggtcacctgg caaaacgacg atcttcttag gggcagacat tagaatggta tatccttgaa
      241 atatatatat atatattgct gaaatgtaaa aggtaagaaa agttagaaag taagacgatt
      301 gctaaccacc tattggaaaa aacaataggt ccttaaataa tattgtcaac ttcaagtatt
      361 gtgatgcaag catttagtca tgaacgcttc tctattctat atgaaaagcc ggttccggcg
      421 ctctcacctt tcctttttct cccaattttt cagttgaaaa aggtatatgc gtcaggcgac
      481 ctctgaaatt aacaaaaaat ttccagtcat cgaatttgat tctgtgcgat agcgcccctg
      541 tgtgttctcg ttatgttgag gaaaaaaata atggttgcta agagattcga actcttgcat
      601 cttacgatac ctgagtattc ccacagttaa ctgcggtcaa gatatttctt gaatcaggcg
      661 ccttagaccg ctcggccaaa caaccaatta cttgttgaga aatagagtat aattatccta
      721 taaatataac gtttttgaac acacatgaac aaggaagtac aggacaattg attttgaaga
      781 gaatgtggat tttgatgtaa ttgttgggat tccattttta ataaggcaat aatattaggt
      841 atgtagatat actagaagtt ctcctcgatg gcgcgcctga tgcggtattt tctccttacg
      901 catctgtgcg gtatttcaca ccgcataggg taataactga tataattaaa ttgaagctct
      961 aatttgtgag tttagtatac atgcatttac ttataataca gttttttagt tttgctggcc
     1021 gcatcttctc aaatatgctt cccagcctgc ttttctgtaa cgttcaccct ctaccttagc
     1081 atcccttccc tttgcaaata gtcctcttcc aacaataata atgtcagatc ctgta
//
